Human PNP ELISA Kit PicoKine®

Purine nucleoside phosphorylase, PNP, PNPase or inosine phosphorylase (EC 2.4.2.1) is an enzyme that in humans is encoded by the NP gene. This gene encodes an enzyme which reversibly catalyzes the phosphorolysis of purine nucleosides. The enzyme is trimeric, containing three identical subunits. Mutations which result in nucleoside phosphorylase deficiency result in defective T-cell (cell-mediated) immunity but can also affect B-cell immunity and antibody responses. Neurologic disorders may also be apparent in patients with immune defects. A known polymorphism at aa position 51 that does not affect enzyme activity has been described. A pseudogene has been identified on chromosome 2.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 36 54 39 56 46 2.38 5.1% Sample 2 117 96 91 114 105 4.92 4.7% Sample 3 639 729 746 580 674 39.27 5.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 36 54 39 56 46 2.38 5.1% Sample 1 36 54 39 56 46 2.38 5.1% Sample 2 117 96 91 114 105 4.92 4.7% Sample 2 117 96 91 114 105 4.92 4.7% Sample 3 639 729 746 580 674 39.27 5.8% Sample 3 639 729 746 580 674 39.27 5.8%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 36 117 639 48 83 827 Standard deviation 2.45 4.25 29.52 3.27 5.07 29.77 CV (%) 6.8% 3.6% 4.6% 6.8% 6.1% 3.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 36 117 639 48 83 827 Standard deviation 2.45 4.25 29.52 3.27 5.07 29.77 CV (%) 6.8% 3.6% 4.6% 6.8% 6.1% 3.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 36 117 639 48 83 827 Mean (pg/ml) 36 117 639 48 83 827 Standard deviation 2.45 4.25 29.52 3.27 5.07 29.77 Standard deviation 2.45 4.25 29.52 3.27 5.07 29.77 CV (%) 6.8% 3.6% 4.6% 6.8% 6.1% 3.6% CV (%) 6.8% 3.6% 4.6% 6.8% 6.1% 3.6%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.042 0.096 0.152 0.251 0.427 0.775 1.373 2.238 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.042 0.096 0.152 0.251 0.427 0.775 1.373 2.238 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.042 0.096 0.152 0.251 0.427 0.775 1.373 2.238 O.D. 0.042 0.096 0.152 0.251 0.427 0.775 1.373 2.238

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.