Human PKLR ELISA Kit PicoKine®

Pyruvate kinase PKLR is an enzyme that in humans is encoded by the PKLR gene. The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA) . Multiple transcript variants encoding different isoforms have been found for this gene.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 185 168 186 177 179 13.75 7.7% Sample 2 335 263 278 311 297 11.07 3.7% Sample 3 2366 1875 2286 2162 2172 144.02 6.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 185 168 186 177 179 13.75 7.7% Sample 1 185 168 186 177 179 13.75 7.7% Sample 2 335 263 278 311 297 11.07 3.7% Sample 2 335 263 278 311 297 11.07 3.7% Sample 3 2366 1875 2286 2162 2172 144.02 6.6% Sample 3 2366 1875 2286 2162 2172 144.02 6.6%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 185 335 2366 185 267 1851 Standard deviation 7.25 13.97 57.97 8.94 18.42 116.8 CV (%) 3.9% 4.2% 2.5% 4.8% 6.9% 6.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 185 335 2366 185 267 1851 Standard deviation 7.25 13.97 57.97 8.94 18.42 116.8 CV (%) 3.9% 4.2% 2.5% 4.8% 6.9% 6.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 185 335 2366 185 267 1851 Mean (pg/ml) 185 335 2366 185 267 1851 Standard deviation 7.25 13.97 57.97 8.94 18.42 116.8 Standard deviation 7.25 13.97 57.97 8.94 18.42 116.8 CV (%) 3.9% 4.2% 2.5% 4.8% 6.9% 6.3% CV (%) 3.9% 4.2% 2.5% 4.8% 6.9% 6.3%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.026 0.116 0.197 0.338 0.557 1.066 1.565 2.056 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.026 0.116 0.197 0.338 0.557 1.066 1.565 2.056 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.026 0.116 0.197 0.338 0.557 1.066 1.565 2.056 O.D. 0.026 0.116 0.197 0.338 0.557 1.066 1.565 2.056

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.