Human PILRB ELISA Kit PicoKine®

Paired immunoglobulin-like type 2 receptor beta is a protein that in humans is encoded by the PILRB gene. The paired immunoglobin-like type 2 receptors consist of highly related activating and inhibitory receptors that are involved in the regulation of many aspects of the immune system. The paired immunoglobulin-like receptor genes are located in a tandem head-to-tail orientation on chromosome 7. This gene encodes the activating member of the receptor pair and contains a truncated cytoplasmic tail relative to its inhibitory counterpart (PILRA), that has a long cytoplasmic tail with immunoreceptor tyrosine-based inhibitory (ITIM) motifs. This gene is thought to have arisen from a duplication of the inhibitory PILRA gene and evolved to acquire its activating function.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 116 101 134 148 125 9.16 7.3% Sample 2 321 303 263 351 310 19.07 6.2% Sample 3 2663 1678 2544 1933 2205 141.31 6.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 116 101 134 148 125 9.16 7.3% Sample 1 116 101 134 148 125 9.16 7.3% Sample 2 321 303 263 351 310 19.07 6.2% Sample 2 321 303 263 351 310 19.07 6.2% Sample 3 2663 1678 2544 1933 2205 141.31 6.4% Sample 3 2663 1678 2544 1933 2205 141.31 6.4%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 116 321 2663 143 371 2601 Standard deviation 9.2 16.15 136.88 3.47 9.35 99.62 CV (%) 7.9% 5% 5.1% 2.4% 2.5% 3.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 116 321 2663 143 371 2601 Standard deviation 9.2 16.15 136.88 3.47 9.35 99.62 CV (%) 7.9% 5% 5.1% 2.4% 2.5% 3.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 116 321 2663 143 371 2601 Mean (pg/ml) 116 321 2663 143 371 2601 Standard deviation 9.2 16.15 136.88 3.47 9.35 99.62 Standard deviation 9.2 16.15 136.88 3.47 9.35 99.62 CV (%) 7.9% 5% 5.1% 2.4% 2.5% 3.8% CV (%) 7.9% 5% 5.1% 2.4% 2.5% 3.8%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.015 0.079 0.148 0.283 0.583 0.992 1.632 1.961 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.015 0.079 0.148 0.283 0.583 0.992 1.632 1.961 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.015 0.079 0.148 0.283 0.583 0.992 1.632 1.961 O.D. 0.015 0.079 0.148 0.283 0.583 0.992 1.632 1.961

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.