Human PILRA ELISA Kit PicoKine®

Paired immunoglobin like type 2 receptor alpha is a protein that in humans is encoded by the PILRA gene. Cell signaling pathways rely on a dynamic interaction between activating and inhibiting processes. SHP-1-mediated dephosphorylation of protein tyrosine residues is central to the regulation of several cell signaling pathways. Two types of inhibitory receptor superfamily members are immunoreceptor tyrosine-based inhibitory motif (ITIM) -bearing receptors and their non-ITIM-bearing, activating counterparts. Control of cell signaling via SHP-1 is thought to occur through a balance between PILRalpha-mediated inhibition and PILRbeta-mediated activation. These paired immunoglobulin-like receptor genes are located in a tandem head-to-tail orientation on chromosome 7. This particular gene encodes the ITIM-bearing member of the receptor pair, which functions in the inhibitory role. Alternative splicing has been observed at this locus and three variants, each encoding a distinct isoform, are described.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 218 179 311 244 238 6.24 2.6% Sample 2 352 461 582 429 456 16.51 3.6% Sample 3 3904 4411 4702 3842 4215 170.28 4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 218 179 311 244 238 6.24 2.6% Sample 1 218 179 311 244 238 6.24 2.6% Sample 2 352 461 582 429 456 16.51 3.6% Sample 2 352 461 582 429 456 16.51 3.6% Sample 3 3904 4411 4702 3842 4215 170.28 4% Sample 3 3904 4411 4702 3842 4215 170.28 4%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 218 352 3904 293 473 2628 Standard deviation 14.54 25.73 169.82 13.33 25.26 181.07 CV (%) 6.7% 7.3% 4.4% 4.6% 5.3% 6.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 218 352 3904 293 473 2628 Standard deviation 14.54 25.73 169.82 13.33 25.26 181.07 CV (%) 6.7% 7.3% 4.4% 4.6% 5.3% 6.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 218 352 3904 293 473 2628 Mean (pg/ml) 218 352 3904 293 473 2628 Standard deviation 14.54 25.73 169.82 13.33 25.26 181.07 Standard deviation 14.54 25.73 169.82 13.33 25.26 181.07 CV (%) 6.7% 7.3% 4.4% 4.6% 5.3% 6.9% CV (%) 6.7% 7.3% 4.4% 4.6% 5.3% 6.9%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.032 0.15 0.276 0.429 0.762 1.189 1.684 2.022 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.032 0.15 0.276 0.429 0.762 1.189 1.684 2.022 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.032 0.15 0.276 0.429 0.762 1.189 1.684 2.022 O.D. 0.032 0.15 0.276 0.429 0.762 1.189 1.684 2.022

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.