Human IL-17D ELISA Kit PicoKine®

Interleukin 17D (IL-17D) is a member of the unique IL-17 family, which share a conserved cysteine motif. It is mapped to 13q12.11. IL-17D regulates cytokine production in endothelial cells and inhibits hemopoiesis in vitro. Purified recombinant IL-17D inhibited myeloid progenitor cell colony formation. IL-17D can enhance endothelial cell production of IL6, IL8, and GMCSF, but not other cytokines. And it is preferentially expressed in skeletal muscle, brain, adipose tissue, heart, lung, and pancreas.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 67 66 88 67 72 1.54 2.1% Sample 2 157 185 189 140 168 9.96 5.7% Sample 3 1226 1378 1711 1846 1540 74.09 4.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 67 66 88 67 72 1.54 2.1% Sample 1 67 66 88 67 72 1.54 2.1% Sample 2 157 185 189 140 168 9.96 5.7% Sample 2 157 185 189 140 168 9.96 5.7% Sample 3 1226 1378 1711 1846 1540 74.09 4.8% Sample 3 1226 1378 1711 1846 1540 74.09 4.8%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 67 157 1226 106 191 1576 Standard deviation 4.47 8.04 25.62 2.87 11.29 71.87 CV (%) 6.7% 5.1% 2.1% 2.7% 5.9% 4.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 67 157 1226 106 191 1576 Standard deviation 4.47 8.04 25.62 2.87 11.29 71.87 CV (%) 6.7% 5.1% 2.1% 2.7% 5.9% 4.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 67 157 1226 106 191 1576 Mean (pg/ml) 67 157 1226 106 191 1576 Standard deviation 4.47 8.04 25.62 2.87 11.29 71.87 Standard deviation 4.47 8.04 25.62 2.87 11.29 71.87 CV (%) 6.7% 5.1% 2.1% 2.7% 5.9% 4.6% CV (%) 6.7% 5.1% 2.1% 2.7% 5.9% 4.6%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.033 0.149 0.26 0.45 0.784 1.193 1.695 1.909 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.033 0.149 0.26 0.45 0.784 1.193 1.695 1.909 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.033 0.149 0.26 0.45 0.784 1.193 1.695 1.909 O.D. 0.033 0.149 0.26 0.45 0.784 1.193 1.695 1.909

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.