Human LIF ELISA Kit PicoKine®

Leukemia inhibitory factor, or LIF, is an interleukin 6 class cytokine that affects cell growth by inhibiting differentiation. When LIF levels drop, the cells differentiate. The protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 23 27 16 16 21 1.12 5.5% Sample 2 40 51 32 47 43 3.2 7.5% Sample 3 341 484 449 426 425 27.71 6.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 23 27 16 16 21 1.12 5.5% Sample 1 23 27 16 16 21 1.12 5.5% Sample 2 40 51 32 47 43 3.2 7.5% Sample 2 40 51 32 47 43 3.2 7.5% Sample 3 341 484 449 426 425 27.71 6.5% Sample 3 341 484 449 426 425 27.71 6.5%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 23 40 341 30 54 431 Standard deviation 0.6 2.14 18.96 0.79 1.73 18.02 CV (%) 2.6% 5.3% 5.6% 2.6% 3.2% 4.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 23 40 341 30 54 431 Standard deviation 0.6 2.14 18.96 0.79 1.73 18.02 CV (%) 2.6% 5.3% 5.6% 2.6% 3.2% 4.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 23 40 341 30 54 431 Mean (pg/ml) 23 40 341 30 54 431 Standard deviation 0.6 2.14 18.96 0.79 1.73 18.02 Standard deviation 0.6 2.14 18.96 0.79 1.73 18.02 CV (%) 2.6% 5.3% 5.6% 2.6% 3.2% 4.2% CV (%) 2.6% 5.3% 5.6% 2.6% 3.2% 4.2%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.033 0.111 0.179 0.301 0.574 0.89 1.5 2.085 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.033 0.111 0.179 0.301 0.574 0.89 1.5 2.085 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.033 0.111 0.179 0.301 0.574 0.89 1.5 2.085 O.D. 0.033 0.111 0.179 0.301 0.574 0.89 1.5 2.085

Activate the sample Serum, plasma (EDTA) : add activating reagent pro rata, i.e. add 20μl of Solution A into 40μl of sample, 10 min later, add 20μl of Solution B. PH 7.0-7.6. It is unnecessary to activate the Cell culture supernate. It is unnecessary to activate the recombinant LIF. Sample was diluted partly after adding activating reagent, so please pay attention to this when calculate target protein concentration.

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.