Human MSR ELISA Kit PicoKine®

Macrophage scavenger receptor 1, also known as MSR1, is a protein which in humans is encoded by the MSR1 gene. This gene encodes the class A macrophage scavenger receptors, which include three different types (1, 2, 3) generated by alternative splicing of this gene. These receptors or isoforms are macrophage-specific trimeric integral membrane glycoproteins and have been implicated in many macrophage-associated physiological and pathological processes including atherosclerosis, Alzheimer's disease, and host defense. The isoforms type 1 and type 2 are functional receptors and are able to mediate the endocytosis of modified low density lipoproteins (LDLs) . The isoform type 3 does not internalize modified LDL (acetyl-LDL) despite having the domain shown to mediate this function in the types 1 and 2 isoforms. It has an altered intracellular processing and is trapped within the endoplasmic reticulum, making it unable to perform endocytosis. The isoform type 3 can inhibit the function of isoforms type 1 and type 2 when co-expressed, indicating a dominant negative effect and suggesting a mechanism for regulation of scavenger receptor activity in macrophages.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 116 184 179 106 146 3.69 2.5% Sample 2 302 281 209 256 262 9.62 3.7% Sample 3 2899 2065 2610 2707 2570 55.52 2.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 116 184 179 106 146 3.69 2.5% Sample 1 116 184 179 106 146 3.69 2.5% Sample 2 302 281 209 256 262 9.62 3.7% Sample 2 302 281 209 256 262 9.62 3.7% Sample 3 2899 2065 2610 2707 2570 55.52 2.2% Sample 3 2899 2065 2610 2707 2570 55.52 2.2%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 116 302 2899 149 191 2452 Standard deviation 4.04 23.62 198.87 9.15 12.45 179.73 CV (%) 3.5% 7.8% 6.9% 6.1% 6.5% 7.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 116 302 2899 149 191 2452 Standard deviation 4.04 23.62 198.87 9.15 12.45 179.73 CV (%) 3.5% 7.8% 6.9% 6.1% 6.5% 7.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 116 302 2899 149 191 2452 Mean (pg/ml) 116 302 2899 149 191 2452 Standard deviation 4.04 23.62 198.87 9.15 12.45 179.73 Standard deviation 4.04 23.62 198.87 9.15 12.45 179.73 CV (%) 3.5% 7.8% 6.9% 6.1% 6.5% 7.3% CV (%) 3.5% 7.8% 6.9% 6.1% 6.5% 7.3%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.012 0.092 0.153 0.285 0.572 0.924 1.469 2.004 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.012 0.092 0.153 0.285 0.572 0.924 1.469 2.004 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.012 0.092 0.153 0.285 0.572 0.924 1.469 2.004 O.D. 0.012 0.092 0.153 0.285 0.572 0.924 1.469 2.004

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.