Human PDGFRB ELISA Kit PicoKine®

Platelet-derived growth factor receptor beta is a protein that in humans is encoded by the PDGFRB gene. The protein encoded by this gene is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer (PDGFB or PDGFD) or a heterodimer (PDGFA and PDGFB) . This gene is essential for normal development of the cardiovascular system and aids in rearrangement of the actin cytoskeleton. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the ETV6 gene, results in chronic myeloproliferative disorder with eosinophilia.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 263 268 172 274 244 9.09 3.7% Sample 2 497 618 539 507 540 21.5 4% Sample 3 3230 3108 3988 2566 3223 194.35 6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 263 268 172 274 244 9.09 3.7% Sample 1 263 268 172 274 244 9.09 3.7% Sample 2 497 618 539 507 540 21.5 4% Sample 2 497 618 539 507 540 21.5 4% Sample 3 3230 3108 3988 2566 3223 194.35 6% Sample 3 3230 3108 3988 2566 3223 194.35 6%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 263 497 3230 268 580 3309 Standard deviation 7.18 18.69 69.42 8.58 30.91 218.72 CV (%) 2.7% 3.8% 2% 3.2% 5.3% 6.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 263 497 3230 268 580 3309 Standard deviation 7.18 18.69 69.42 8.58 30.91 218.72 CV (%) 2.7% 3.8% 2% 3.2% 5.3% 6.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 263 497 3230 268 580 3309 Mean (pg/ml) 263 497 3230 268 580 3309 Standard deviation 7.18 18.69 69.42 8.58 30.91 218.72 Standard deviation 7.18 18.69 69.42 8.58 30.91 218.72 CV (%) 2.7% 3.8% 2% 3.2% 5.3% 6.6% CV (%) 2.7% 3.8% 2% 3.2% 5.3% 6.6%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.004 0.122 0.239 0.405 0.72 1.15 1.64 2.007 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.004 0.122 0.239 0.405 0.72 1.15 1.64 2.007 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.004 0.122 0.239 0.405 0.72 1.15 1.64 2.007 O.D. 0.004 0.122 0.239 0.405 0.72 1.15 1.64 2.007

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.