Human NCR2 ELISA Kit PicoKine®

Human NCR2 PicoKine® ELISA Kit (96 Tests) . Quantitate Human NCR2 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 25 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Natural cytotoxicity triggering receptor 2 is a protein that in humans is encoded by the NCR2 gene. Predicted to enable signaling receptor activity. Predicted to be involved in cellular defense response and signal transduction. Predicted to be located in plasma membrane. Predicted to be integral component of plasma membrane. Predicted to be active in cell surface.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 82 89 65 69 76 2.14 2.8% Sample 2 109 112 173 168 141 4 2.9% Sample 3 1149 1259 1052 1227 1172 77.57 6.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 82 89 65 69 76 2.14 2.8% Sample 1 82 89 65 69 76 2.14 2.8% Sample 2 109 112 173 168 141 4 2.9% Sample 2 109 112 173 168 141 4 2.9% Sample 3 1149 1259 1052 1227 1172 77.57 6.6% Sample 3 1149 1259 1052 1227 1172 77.57 6.6%

Gene Name

NCR2

Gene ID

9436

UniProt

O95944

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: CHO; Immunogen sequence: Q22-P190

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA)

Detection Range

46.9 pg/mL - 3,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 82 109 1149 72 113 824 Standard deviation 2.76 8.57 86.06 1.82 2.94 22.82 CV (%) 3.4% 7.9% 7.5% 2.5% 2.6% 2.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 82 109 1149 72 113 824 Standard deviation 2.76 8.57 86.06 1.82 2.94 22.82 CV (%) 3.4% 7.9% 7.5% 2.5% 2.6% 2.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 82 109 1149 72 113 824 Mean (pg/ml) 82 109 1149 72 113 824 Standard deviation 2.76 8.57 86.06 1.82 2.94 22.82 Standard deviation 2.76 8.57 86.06 1.82 2.94 22.82 CV (%) 3.4% 7.9% 7.5% 2.5% 2.6% 2.8% CV (%) 3.4% 7.9% 7.5% 2.5% 2.6% 2.8%

Sensitivity

<25 pg/mL

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human NCR2

Anticoagulant

Heparin or EDTA

Sample Data

Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.006 0.047 0.077 0.158 0.294 0.615 1.166 2.047 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.006 0.047 0.077 0.158 0.294 0.615 1.166 2.047 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.006 0.047 0.077 0.158 0.294 0.615 1.166 2.047 O.D. 0.006 0.047 0.077 0.158 0.294 0.615 1.166 2.047

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.