Human Napsin A ELISA Kit PicoKine®

Napsin-A is an aspartic proteinase that is encoded in humans by the NAPSA gene. This gene encodes a member of the peptidase A1 family of aspartic proteases. The encoded preproprotein is proteolytically processed to generate an activation peptide and the mature protease. The activation peptides of aspartic proteinases function as inhibitors of the protease active site. These peptide segments, or pro-parts, are deemed important for correct folding, targeting, and control of the activation of aspartic proteinase zymogens. The encoded protease may play a role in the proteolytic processing of pulmonary surfactant protein B in the lung and may function in protein catabolism in the renal proximal tubules. This gene has been described as a marker for lung adenocarcinoma and renal cell carcinoma.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 106 77 106 78 92 3.9 4.3% Sample 2 154 209 182 247 198 7.78 3.9% Sample 3 1205 1979 1152 1246 1396 66.15 4.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 106 77 106 78 92 3.9 4.3% Sample 1 106 77 106 78 92 3.9 4.3% Sample 2 154 209 182 247 198 7.78 3.9% Sample 2 154 209 182 247 198 7.78 3.9% Sample 3 1205 1979 1152 1246 1396 66.15 4.7% Sample 3 1205 1979 1152 1246 1396 66.15 4.7%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 106 154 1205 82 237 1482 Standard deviation 3.93 3.26 50.49 3.62 18.94 40.61 CV (%) 3.7% 2.1% 4.2% 4.4% 8% 2.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 106 154 1205 82 237 1482 Standard deviation 3.93 3.26 50.49 3.62 18.94 40.61 CV (%) 3.7% 2.1% 4.2% 4.4% 8% 2.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 106 154 1205 82 237 1482 Mean (pg/ml) 106 154 1205 82 237 1482 Standard deviation 3.93 3.26 50.49 3.62 18.94 40.61 Standard deviation 3.93 3.26 50.49 3.62 18.94 40.61 CV (%) 3.7% 2.1% 4.2% 4.4% 8% 2.7% CV (%) 3.7% 2.1% 4.2% 4.4% 8% 2.7%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.052 0.101 0.175 0.317 0.608 0.955 1.52 2.048 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.052 0.101 0.175 0.317 0.608 0.955 1.52 2.048 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.052 0.101 0.175 0.317 0.608 0.955 1.52 2.048 O.D. 0.052 0.101 0.175 0.317 0.608 0.955 1.52 2.048

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.