Human NAGA ELISA Kit PicoKine®

NAGA encodes the lysosomal enzyme alpha-N-acetylgalactosaminidase, which cleaves alpha-N-acetylgalactosaminyl moieties from glycoconjugates. Mutations in NAGA have been identified as the cause of Schindler disease types I and II (type II also known as Kanzaki disease) .

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 23 20 26 29 25 1.04 4.2% Sample 2 49 56 33 53 48 3.18 6.7% Sample 3 424 272 483 282 365 28.75 7.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 23 20 26 29 25 1.04 4.2% Sample 1 23 20 26 29 25 1.04 4.2% Sample 2 49 56 33 53 48 3.18 6.7% Sample 2 49 56 33 53 48 3.18 6.7% Sample 3 424 272 483 282 365 28.75 7.9% Sample 3 424 272 483 282 365 28.75 7.9%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 23 49 424 22 59 327 Standard deviation 1.49 1.41 16.03 0.58 3.36 22.76 CV (%) 6.5% 2.9% 3.8% 2.6% 5.7% 7.0% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 23 49 424 22 59 327 Standard deviation 1.49 1.41 16.03 0.58 3.36 22.76 CV (%) 6.5% 2.9% 3.8% 2.6% 5.7% 7.0% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 23 49 424 22 59 327 Mean (pg/ml) 23 49 424 22 59 327 Standard deviation 1.49 1.41 16.03 0.58 3.36 22.76 Standard deviation 1.49 1.41 16.03 0.58 3.36 22.76 CV (%) 6.5% 2.9% 3.8% 2.6% 5.7% 7.0% CV (%) 6.5% 2.9% 3.8% 2.6% 5.7% 7.0%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.056 0.097 0.138 0.206 0.4 0.7 1.19 1.889 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.056 0.097 0.138 0.206 0.4 0.7 1.19 1.889 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.056 0.097 0.138 0.206 0.4 0.7 1.19 1.889 O.D. 0.056 0.097 0.138 0.206 0.4 0.7 1.19 1.889

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.