Human NAGA ELISA Kit PicoKine®

Human NAGA PicoKine® ELISA Kit (96 Tests) . Quantitate Human NAGA in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 8 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

NAGA encodes the lysosomal enzyme alpha-N-acetylgalactosaminidase, which cleaves alpha-N-acetylgalactosaminyl moieties from glycoconjugates. Mutations in NAGA have been identified as the cause of Schindler disease types I and II (type II also known as Kanzaki disease) .

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 23 20 26 29 25 1.04 4.2% Sample 2 49 56 33 53 48 3.18 6.7% Sample 3 424 272 483 282 365 28.75 7.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 23 20 26 29 25 1.04 4.2% Sample 1 23 20 26 29 25 1.04 4.2% Sample 2 49 56 33 53 48 3.18 6.7% Sample 2 49 56 33 53 48 3.18 6.7% Sample 3 424 272 483 282 365 28.75 7.9% Sample 3 424 272 483 282 365 28.75 7.9%

Gene Name

NAGA

Gene ID

4668

UniProt

P17050

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: CHO; Immunogen sequence: L18-Q411

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA)

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 23 49 424 22 59 327 Standard deviation 1.49 1.41 16.03 0.58 3.36 22.76 CV (%) 6.5% 2.9% 3.8% 2.6% 5.7% 7.0% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 23 49 424 22 59 327 Standard deviation 1.49 1.41 16.03 0.58 3.36 22.76 CV (%) 6.5% 2.9% 3.8% 2.6% 5.7% 7.0% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 23 49 424 22 59 327 Mean (pg/ml) 23 49 424 22 59 327 Standard deviation 1.49 1.41 16.03 0.58 3.36 22.76 Standard deviation 1.49 1.41 16.03 0.58 3.36 22.76 CV (%) 6.5% 2.9% 3.8% 2.6% 5.7% 7.0% CV (%) 6.5% 2.9% 3.8% 2.6% 5.7% 7.0%

Sensitivity

<8 pg/mL

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human NAGA

Anticoagulant

Heparin or EDTA

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.056 0.097 0.138 0.206 0.4 0.7 1.19 1.889 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.056 0.097 0.138 0.206 0.4 0.7 1.19 1.889 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.056 0.097 0.138 0.206 0.4 0.7 1.19 1.889 O.D. 0.056 0.097 0.138 0.206 0.4 0.7 1.19 1.889

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.