Human ASAHL ELISA Kit PicoKine®

N-acylethanolamine-hydrolyzing acid amidase is an enzyme that in humans is encoded by the NAAA gene. Enables N- (long-chain-acyl) ethanolamine deacylase activity; N-acylsphingosine amidohydrolase activity; and fatty acid amide hydrolase activity. Involved in several processes, including N-acylethanolamine metabolic process; N-acylphosphatidylethanolamine metabolic process; and sphingosine metabolic process. Located in lysosome. Is extrinsic component of membrane.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 30 30 24 25 27 1.71 6.3% Sample 2 48 36 49 59 48 2.75 5.7% Sample 3 437 401 423 452 428 22.18 5.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 30 30 24 25 27 1.71 6.3% Sample 1 30 30 24 25 27 1.71 6.3% Sample 2 48 36 49 59 48 2.75 5.7% Sample 2 48 36 49 59 48 2.75 5.7% Sample 3 437 401 423 452 428 22.18 5.2% Sample 3 437 401 423 452 428 22.18 5.2%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 30 48 437 25 34 452 Standard deviation 0.88 2.37 31.81 0.84 1.17 12.75 CV (%) 2.9% 4.9% 7.3% 3.3% 3.5% 2.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 30 48 437 25 34 452 Standard deviation 0.88 2.37 31.81 0.84 1.17 12.75 CV (%) 2.9% 4.9% 7.3% 3.3% 3.5% 2.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 30 48 437 25 34 452 Mean (pg/ml) 30 48 437 25 34 452 Standard deviation 0.88 2.37 31.81 0.84 1.17 12.75 Standard deviation 0.88 2.37 31.81 0.84 1.17 12.75 CV (%) 2.9% 4.9% 7.3% 3.3% 3.5% 2.8% CV (%) 2.9% 4.9% 7.3% 3.3% 3.5% 2.8%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.046 0.145 0.215 0.359 0.6116 1.097 1.792 2.381 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.046 0.145 0.215 0.359 0.6116 1.097 1.792 2.381 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.046 0.145 0.215 0.359 0.6116 1.097 1.792 2.381 O.D. 0.046 0.145 0.215 0.359 0.6116 1.097 1.792 2.381

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.