Human ASAHL ELISA Kit PicoKine®

Human ASAHL PicoKine® ® Quick ELISA Kit PicoKine® ELISA Kit (96 Tests) . Quantitate Human ASAHL in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 8 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

N-acylethanolamine-hydrolyzing acid amidase is an enzyme that in humans is encoded by the NAAA gene. Enables N- (long-chain-acyl) ethanolamine deacylase activity; N-acylsphingosine amidohydrolase activity; and fatty acid amide hydrolase activity. Involved in several processes, including N-acylethanolamine metabolic process; N-acylphosphatidylethanolamine metabolic process; and sphingosine metabolic process. Located in lysosome. Is extrinsic component of membrane.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 30 30 24 25 27 1.71 6.3% Sample 2 48 36 49 59 48 2.75 5.7% Sample 3 437 401 423 452 428 22.18 5.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 30 30 24 25 27 1.71 6.3% Sample 1 30 30 24 25 27 1.71 6.3% Sample 2 48 36 49 59 48 2.75 5.7% Sample 2 48 36 49 59 48 2.75 5.7% Sample 3 437 401 423 452 428 22.18 5.2% Sample 3 437 401 423 452 428 22.18 5.2%

Gene Name

NAAA

Gene ID

27163

UniProt

Q02083

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: CHO; Immunogen sequence: M1-K359

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate)

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 30 48 437 25 34 452 Standard deviation 0.88 2.37 31.81 0.84 1.17 12.75 CV (%) 2.9% 4.9% 7.3% 3.3% 3.5% 2.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 30 48 437 25 34 452 Standard deviation 0.88 2.37 31.81 0.84 1.17 12.75 CV (%) 2.9% 4.9% 7.3% 3.3% 3.5% 2.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 30 48 437 25 34 452 Mean (pg/ml) 30 48 437 25 34 452 Standard deviation 0.88 2.37 31.81 0.84 1.17 12.75 Standard deviation 0.88 2.37 31.81 0.84 1.17 12.75 CV (%) 2.9% 4.9% 7.3% 3.3% 3.5% 2.8% CV (%) 2.9% 4.9% 7.3% 3.3% 3.5% 2.8%

Sensitivity

<8 pg/mL

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human NAAA

Anticoagulant

Heparin, EDTA or citrate

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.046 0.145 0.215 0.359 0.6116 1.097 1.792 2.381 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.046 0.145 0.215 0.359 0.6116 1.097 1.792 2.381 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.046 0.145 0.215 0.359 0.6116 1.097 1.792 2.381 O.D. 0.046 0.145 0.215 0.359 0.6116 1.097 1.792 2.381

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.