Human IL22BP ELISA Kit PicoKine®

This gene encodes a member of the class II cytokine receptor family. The encoded soluble protein specifically binds to and inhibits interleukin 22 activity by blocking the interaction of interleukin 22 with its cell surface receptor. The encoded protein may be important in the regulation of inflammatory response, and has been implicated in the regulation of tumorigenesis in the colon. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 54 65 64 81 66 1.6 2.4% Sample 2 151 158 136 134 145 2.92 2% Sample 3 1130 1499 964 795 1097 30.06 2.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 54 65 64 81 66 1.6 2.4% Sample 1 54 65 64 81 66 1.6 2.4% Sample 2 151 158 136 134 145 2.92 2% Sample 2 151 158 136 134 145 2.92 2% Sample 3 1130 1499 964 795 1097 30.06 2.7% Sample 3 1130 1499 964 795 1097 30.06 2.7%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 54 151 1130 58 130 1247 Standard deviation 2.11 6.67 44.41 1.35 5.34 39.65 CV (%) 3.9% 4.4% 3.9% 2.3% 4.1% 3.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 54 151 1130 58 130 1247 Standard deviation 2.11 6.67 44.41 1.35 5.34 39.65 CV (%) 3.9% 4.4% 3.9% 2.3% 4.1% 3.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 54 151 1130 58 130 1247 Mean (pg/ml) 54 151 1130 58 130 1247 Standard deviation 2.11 6.67 44.41 1.35 5.34 39.65 Standard deviation 2.11 6.67 44.41 1.35 5.34 39.65 CV (%) 3.9% 4.4% 3.9% 2.3% 4.1% 3.2% CV (%) 3.9% 4.4% 3.9% 2.3% 4.1% 3.2%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.034 0.114 0.16 0.275 0.458 0.844 1.468 2.129 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.034 0.114 0.16 0.275 0.458 0.844 1.468 2.129 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.034 0.114 0.16 0.275 0.458 0.844 1.468 2.129 O.D. 0.034 0.114 0.16 0.275 0.458 0.844 1.468 2.129

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.