Human Layilin ELISA Kit PicoKine®

Layilin is a protein that in humans is encoded by the LAYN gene. Layilin is a type I transmembrane protein that acts as a receptor for hyaluronan. It has an extracellular C-type lectin domain and a 123 aa cytoplasmic domain that interacts with the cytoskeleton. The amino acid sequence of recombinant human Layilin is 85%, 82%, 80%, 79% and 76% identical to that of hamster, canine, bovine, mouse and rat.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 101 76 96 84 89 5.39 6% Sample 2 203 223 242 159 207 5.75 2.8% Sample 3 1553 1311 1828 1209 1475 42.04 2.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 101 76 96 84 89 5.39 6% Sample 1 101 76 96 84 89 5.39 6% Sample 2 203 223 242 159 207 5.75 2.8% Sample 2 203 223 242 159 207 5.75 2.8% Sample 3 1553 1311 1828 1209 1475 42.04 2.9% Sample 3 1553 1311 1828 1209 1475 42.04 2.9%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 101 203 1553 64 178 1261 Standard deviation 2.44 7.37 68.49 1.4 7.42 25.47 CV (%) 2.4% 3.6% 4.4% 2.2% 4.2% 2.0% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 101 203 1553 64 178 1261 Standard deviation 2.44 7.37 68.49 1.4 7.42 25.47 CV (%) 2.4% 3.6% 4.4% 2.2% 4.2% 2.0% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 101 203 1553 64 178 1261 Mean (pg/ml) 101 203 1553 64 178 1261 Standard deviation 2.44 7.37 68.49 1.4 7.42 25.47 Standard deviation 2.44 7.37 68.49 1.4 7.42 25.47 CV (%) 2.4% 3.6% 4.4% 2.2% 4.2% 2.0% CV (%) 2.4% 3.6% 4.4% 2.2% 4.2% 2.0%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.02 0.072 0.133 0.268 0.462 0.859 1.461 2.152 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.02 0.072 0.133 0.268 0.462 0.859 1.461 2.152 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.02 0.072 0.133 0.268 0.462 0.859 1.461 2.152 O.D. 0.02 0.072 0.133 0.268 0.462 0.859 1.461 2.152

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.