Human Layilin ELISA Kit PicoKine®

Human Layilin PicoKine® ELISA Kit (96 Tests) . Quantitate Human Layilin in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 30 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Layilin is a protein that in humans is encoded by the LAYN gene. Layilin is a type I transmembrane protein that acts as a receptor for hyaluronan. It has an extracellular C-type lectin domain and a 123 aa cytoplasmic domain that interacts with the cytoskeleton. The amino acid sequence of recombinant human Layilin is 85%, 82%, 80%, 79% and 76% identical to that of hamster, canine, bovine, mouse and rat.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 101 76 96 84 89 5.39 6% Sample 2 203 223 242 159 207 5.75 2.8% Sample 3 1553 1311 1828 1209 1475 42.04 2.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 101 76 96 84 89 5.39 6% Sample 1 101 76 96 84 89 5.39 6% Sample 2 203 223 242 159 207 5.75 2.8% Sample 2 203 223 242 159 207 5.75 2.8% Sample 3 1553 1311 1828 1209 1475 42.04 2.9% Sample 3 1553 1311 1828 1209 1475 42.04 2.9%

Gene Name

LAYN

Gene ID

143903

UniProt

Q6UX15

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A22-E220

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA)

Detection Range

62.5 pg/mL - 4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 101 203 1553 64 178 1261 Standard deviation 2.44 7.37 68.49 1.4 7.42 25.47 CV (%) 2.4% 3.6% 4.4% 2.2% 4.2% 2.0% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 101 203 1553 64 178 1261 Standard deviation 2.44 7.37 68.49 1.4 7.42 25.47 CV (%) 2.4% 3.6% 4.4% 2.2% 4.2% 2.0% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 101 203 1553 64 178 1261 Mean (pg/ml) 101 203 1553 64 178 1261 Standard deviation 2.44 7.37 68.49 1.4 7.42 25.47 Standard deviation 2.44 7.37 68.49 1.4 7.42 25.47 CV (%) 2.4% 3.6% 4.4% 2.2% 4.2% 2.0% CV (%) 2.4% 3.6% 4.4% 2.2% 4.2% 2.0%

Sensitivity

<30 pg/mL

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human LAYN

Anticoagulant

Heparin or EDTA

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.02 0.072 0.133 0.268 0.462 0.859 1.461 2.152 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.02 0.072 0.133 0.268 0.462 0.859 1.461 2.152 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.02 0.072 0.133 0.268 0.462 0.859 1.461 2.152 O.D. 0.02 0.072 0.133 0.268 0.462 0.859 1.461 2.152

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.