Human MDGA1 ELISA Kit PicoKine®

This gene encodes a glycosylphosphatidylinositol (GPI) -anchored cell surface glycoprotein that is expressed predominantly in the developing nervous system. In addition to possessing several cell adhesion molecule-like domains, the mature protein has six Ig-like domains, a single fibronectin type III domain, a MAM domain and a C-terminal GPI-anchoring site. Studies in other mammals suggest this protein plays a role in cell adhesion, migration, and axon guidance and, in the developing brain, neuronal migration. In humans, this gene is associated with bipolar disorder and schizophrenia.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 71 96 76 96 85 4.45 5.3% Sample 2 243 212 200 153 202 6.87 3.4% Sample 3 1519 1498 1148 1690 1464 58.55 4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 71 96 76 96 85 4.45 5.3% Sample 1 71 96 76 96 85 4.45 5.3% Sample 2 243 212 200 153 202 6.87 3.4% Sample 2 243 212 200 153 202 6.87 3.4% Sample 3 1519 1498 1148 1690 1464 58.55 4% Sample 3 1519 1498 1148 1690 1464 58.55 4%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 71 243 1519 119 214 1316 Standard deviation 1.98 16.14 70.79 2.86 9.39 52.38 CV (%) 2.8% 6.6% 4.7% 2.4% 4.4% 4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 71 243 1519 119 214 1316 Standard deviation 1.98 16.14 70.79 2.86 9.39 52.38 CV (%) 2.8% 6.6% 4.7% 2.4% 4.4% 4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 71 243 1519 119 214 1316 Mean (pg/ml) 71 243 1519 119 214 1316 Standard deviation 1.98 16.14 70.79 2.86 9.39 52.38 Standard deviation 1.98 16.14 70.79 2.86 9.39 52.38 CV (%) 2.8% 6.6% 4.7% 2.4% 4.4% 4% CV (%) 2.8% 6.6% 4.7% 2.4% 4.4% 4%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.032 0.097 0.148 0.249 0.432 0.784 1.385 2.265 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.032 0.097 0.148 0.249 0.432 0.784 1.385 2.265 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.032 0.097 0.148 0.249 0.432 0.784 1.385 2.265 O.D. 0.032 0.097 0.148 0.249 0.432 0.784 1.385 2.265

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.