Human MARCO ELISA Kit PicoKine®

Macrophage receptor with collagenous structure (MARCO) is a protein that in humans is encoded by the MARCO gene. The protein encoded by this gene is a member of the class A scavenger receptor family and is part of the innate antimicrobial immune system. The protein may bind both Gram-negative and Gram-positive bacteria via an extracellular, C-terminal, scavenger receptor cysteine-rich (SRCR) domain. In addition to short cytoplasmic and transmembrane domains, there is an extracellular spacer domain and a long, extracellular collagenous domain. The protein may form a trimeric molecule by the association of the collagenous domains of three identical polypeptide chains.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 203 256 289 206 239 10.49 4.4% Sample 2 948 1068 823 1021 965 45.55 4.7% Sample 3 2703 4988 3405 2885 3495 114.29 3.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 203 256 289 206 239 10.49 4.4% Sample 1 203 256 289 206 239 10.49 4.4% Sample 2 948 1068 823 1021 965 45.55 4.7% Sample 2 948 1068 823 1021 965 45.55 4.7% Sample 3 2703 4988 3405 2885 3495 114.29 3.3% Sample 3 2703 4988 3405 2885 3495 114.29 3.3%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 203 948 2703 303 878 3050 Standard deviation 6.56 34.51 132.18 14.15 47.32 219.6 CV (%) 3.2% 3.6% 4.9% 4.7% 5.4% 7.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 203 948 2703 303 878 3050 Standard deviation 6.56 34.51 132.18 14.15 47.32 219.6 CV (%) 3.2% 3.6% 4.9% 4.7% 5.4% 7.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 203 948 2703 303 878 3050 Mean (pg/ml) 203 948 2703 303 878 3050 Standard deviation 6.56 34.51 132.18 14.15 47.32 219.6 Standard deviation 6.56 34.51 132.18 14.15 47.32 219.6 CV (%) 3.2% 3.6% 4.9% 4.7% 5.4% 7.2% CV (%) 3.2% 3.6% 4.9% 4.7% 5.4% 7.2%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.036 0.145 0.229 0.394 0.745 1.346 2.061 2.564 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.036 0.145 0.229 0.394 0.745 1.346 2.061 2.564 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.036 0.145 0.229 0.394 0.745 1.346 2.061 2.564 O.D. 0.036 0.145 0.229 0.394 0.745 1.346 2.061 2.564

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.