Human IL-23 ELISA Kit PicoKine®

This gene encodes a subunit of the heterodimeric cytokine interleukin 23 (IL23) . IL23 is composed of this protein and the p40 subunit of interleukin 12 (IL12B) . The receptor of IL23 is formed by the beta 1 subunit of IL12 (IL12RB1) and an IL23 specific subunit, IL23R. Both IL23 and IL12 can activate the transcription activator STAT4, and stimulate the production of interferon-gamma (IFNG) . In contrast to IL12, which acts mainly on naive CD4 (+) T cells, IL23 preferentially acts on memory CD4 (+) T cells.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 280 157 260 211 227 10.62 4.7% Sample 2 519 602 510 606 559 33.22 5.9% Sample 3 4052 4755 3861 3421 4022 259.84 6.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 280 157 260 211 227 10.62 4.7% Sample 1 280 157 260 211 227 10.62 4.7% Sample 2 519 602 510 606 559 33.22 5.9% Sample 2 519 602 510 606 559 33.22 5.9% Sample 3 4052 4755 3861 3421 4022 259.84 6.5% Sample 3 4052 4755 3861 3421 4022 259.84 6.5%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 280 519 4052 195 572 2736 Standard deviation 17.22 14.12 142.63 8.09 15.44 98.5 CV (%) 6.2% 2.7% 3.5% 4.2% 2.7% 3.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 280 519 4052 195 572 2736 Standard deviation 17.22 14.12 142.63 8.09 15.44 98.5 CV (%) 6.2% 2.7% 3.5% 4.2% 2.7% 3.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 280 519 4052 195 572 2736 Mean (pg/ml) 280 519 4052 195 572 2736 Standard deviation 17.22 14.12 142.63 8.09 15.44 98.5 Standard deviation 17.22 14.12 142.63 8.09 15.44 98.5 CV (%) 6.2% 2.7% 3.5% 4.2% 2.7% 3.6% CV (%) 6.2% 2.7% 3.5% 4.2% 2.7% 3.6%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.009 0.11 0.203 0.348 0.568 0.975 1.495 2.001 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.009 0.11 0.203 0.348 0.568 0.975 1.495 2.001 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.009 0.11 0.203 0.348 0.568 0.975 1.495 2.001 O.D. 0.009 0.11 0.203 0.348 0.568 0.975 1.495 2.001

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.