Human LTK ELISA Kit PicoKine®

Human LTK PicoKine® ELISA Kit (96 Tests) . Quantitate Human LTK in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 8 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Leukocyte receptor tyrosine kinase is an enzyme that in humans is encoded by the LTK gene. The protein encoded by this gene is a member of the ros/insulin receptor family of tyrosine kinases. Tyrosine-specific phosphorylation of proteins is a key to the control of diverse pathways leading to cell growth and differentiation. Multiple transcript variants encoding different isoforms have been found for this gene.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 18 26 28 17 22 0.55 2.5% Sample 2 54 58 37 53 51 3.23 6.4% Sample 3 403 483 362 257 376 25.43 6.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 18 26 28 17 22 0.55 2.5% Sample 1 18 26 28 17 22 0.55 2.5% Sample 2 54 58 37 53 51 3.23 6.4% Sample 2 54 58 37 53 51 3.23 6.4% Sample 3 403 483 362 257 376 25.43 6.8% Sample 3 403 483 362 257 376 25.43 6.8%

Gene Name

LTK

Gene ID

4058

UniProt

P29376

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: S20-P424

Capture Antibody

A polyclonal antibody from goat

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA)

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 18 54 403 27 58 348 Standard deviation 0.8 1.76 14.27 1.19 4.39 22.1 CV (%) 4.4% 3.3% 3.5% 4.4% 7.6% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 18 54 403 27 58 348 Standard deviation 0.8 1.76 14.27 1.19 4.39 22.1 CV (%) 4.4% 3.3% 3.5% 4.4% 7.6% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 18 54 403 27 58 348 Mean (pg/ml) 18 54 403 27 58 348 Standard deviation 0.8 1.76 14.27 1.19 4.39 22.1 Standard deviation 0.8 1.76 14.27 1.19 4.39 22.1 CV (%) 4.4% 3.3% 3.5% 4.4% 7.6% 6.4% CV (%) 4.4% 3.3% 3.5% 4.4% 7.6% 6.4%

Sensitivity

<8 pg/mL

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human LTK

Anticoagulant

Heparin or EDTA

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.033 0.159 0.249 0.448 0.785 1.405 1.981 2.319 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.033 0.159 0.249 0.448 0.785 1.405 1.981 2.319 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.033 0.159 0.249 0.448 0.785 1.405 1.981 2.319 O.D. 0.033 0.159 0.249 0.448 0.785 1.405 1.981 2.319

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.