Human LRRTM3 ELISA Kit PicoKine®

Human LRRTM3 (leucine-rich repeat transmembrane neuronal 3) is a 63 kDa (predicted) type I transmembrane protein, and one of four members of the LRRTM family of proteins within the leucine-rich repeat (LRR) superfamily. There are two isoforms of LRRTM3. Isoform 1 is synthesized as a precursor with a 30 amino acid (aa) signal sequence, a 389 aa extracellular region, a 21 aa transmembrane region, and a 141 aa cytoplasmic region. In isoform 2, aa 513 is changed from isoleucine to valine, and aa 514 to 581 are missing, producing a cytoplasmic region of only 74 aa. The extracellular region of both isoforms contains one N-linked glycosylation site, a leucine-rich repeat N-terminal domain bordered by four conserved cysteines, and 10 LRRs flanked by cysteine-rich domains. The cytoplasmic region of both isoforms contains several tyrosine, serine, and threonine residues that have the potential to be phosphorylated, and thus to be involved in signal transduction. The C-terminal of isoform 2 also contains a conserved glutamic acid-cysteine-glutamic acid-valine sequence for potential interaction with PDZ proteins. Mature human LRRTM3 (isoform 1) is 98% identical to mouse LRRTM3. In the mouse, beginning at 8.5 dpc, strong levels of LRRTM3 can be detected in the neural progenitors of the neural plate that will develop into the rostral neural tube, the forebrain, a stripe in the hindbrain, and the region of the presomitic mesoderm/somite boundary. By 15 dpc, LRRTM3 is expressed broadly and accumulates thereafter. In the adult, LRRTM3 is expressed almost exclusively in the brain with high expression in the cortical laminae and dentate gyrus, as well as detectable levels in the hypothalamus and amygdala. Functionally, LRRTM3 may be involved in the formation of the CNS and maintenance of CNS structure and function in the adult brain. In addition, LRRTM3 has been shown to promote processing of amyloid-precursor protein by BACE1, and is a positional candidate gene for late-onset Alzheimer’s disease.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 45 39 47 55 47 2.52 5.4% Sample 2 82 64 114 81 85 6.77 7.9% Sample 3 691 838 565 890 746 18.58 2.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 45 39 47 55 47 2.52 5.4% Sample 1 45 39 47 55 47 2.52 5.4% Sample 2 82 64 114 81 85 6.77 7.9% Sample 2 82 64 114 81 85 6.77 7.9% Sample 3 691 838 565 890 746 18.58 2.5% Sample 3 691 838 565 890 746 18.58 2.5%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 45 82 691 56 114 892 Standard deviation 3.43 6.03 39.53 1.71 2.92 22.66 CV (%) 7.6% 7.4% 5.7% 3.1% 2.6% 2.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 45 82 691 56 114 892 Standard deviation 3.43 6.03 39.53 1.71 2.92 22.66 CV (%) 7.6% 7.4% 5.7% 3.1% 2.6% 2.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 45 82 691 56 114 892 Mean (pg/ml) 45 82 691 56 114 892 Standard deviation 3.43 6.03 39.53 1.71 2.92 22.66 Standard deviation 3.43 6.03 39.53 1.71 2.92 22.66 CV (%) 7.6% 7.4% 5.7% 3.1% 2.6% 2.5% CV (%) 7.6% 7.4% 5.7% 3.1% 2.6% 2.5%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.054 0.14 0.209 0.338 0.552 1.014 1.717 2.931 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.054 0.14 0.209 0.338 0.552 1.014 1.717 2.931 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.054 0.14 0.209 0.338 0.552 1.014 1.717 2.931 O.D. 0.054 0.14 0.209 0.338 0.552 1.014 1.717 2.931

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.