Human LAMP ELISA Kit PicoKine®

This gene encodes a member of the immunoglobulin LAMP, OBCAM and neurotrimin (IgLON) family of proteins. The encoded preproprotein is proteolytically processed to generate a neuronal surface glycoprotein. This protein may act as a selective homophilic adhesion molecule during axon guidance and neuronal growth in the developing limbic system. The encoded protein may also function as a tumor suppressor and may play a role in neuropsychiatric disorders. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 25 17 25 19 22 1.4 6.5% Sample 2 59 53 37 45 49 1.73 3.6% Sample 3 416 381 498 354 412 20.74 5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 25 17 25 19 22 1.4 6.5% Sample 1 25 17 25 19 22 1.4 6.5% Sample 2 59 53 37 45 49 1.73 3.6% Sample 2 59 53 37 45 49 1.73 3.6% Sample 3 416 381 498 354 412 20.74 5% Sample 3 416 381 498 354 412 20.74 5%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 25 59 416 26 55 480 Standard deviation 1.17 3.3 20.55 0.91 3.91 13.3 CV (%) 4.7% 5.6% 4.9% 3.5% 7.1% 2.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 25 59 416 26 55 480 Standard deviation 1.17 3.3 20.55 0.91 3.91 13.3 CV (%) 4.7% 5.6% 4.9% 3.5% 7.1% 2.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 25 59 416 26 55 480 Mean (pg/ml) 25 59 416 26 55 480 Standard deviation 1.17 3.3 20.55 0.91 3.91 13.3 Standard deviation 1.17 3.3 20.55 0.91 3.91 13.3 CV (%) 4.7% 5.6% 4.9% 3.5% 7.1% 2.8% CV (%) 4.7% 5.6% 4.9% 3.5% 7.1% 2.8%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.051 0.109 0.166 0.286 0.488 0.869 1.485 2.258 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.051 0.109 0.166 0.286 0.488 0.869 1.485 2.258 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.051 0.109 0.166 0.286 0.488 0.869 1.485 2.258 O.D. 0.051 0.109 0.166 0.286 0.488 0.869 1.485 2.258

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.