Human LPL ELISA Kit PicoKine®

LPL encodes lipoprotein lipase, which is expressed in heart, muscle, and adipose tissue. LPL functions as a homodimer, and has the dual functions of triglyceride hydrolase and ligand/bridging factor for receptor-mediated lipoprotein uptake. Severe mutations that cause LPL deficiency result in type I hyperlipoproteinemia, while less extreme mutations in LPL are linked to many disorders of lipoprotein metabolism.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 137 100 85 132 114 3.71 3.3% Sample 2 201 296 166 236 225 4.72 2.1% Sample 3 2403 2329 2427 2205 2341 163.17 7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 137 100 85 132 114 3.71 3.3% Sample 1 137 100 85 132 114 3.71 3.3% Sample 2 201 296 166 236 225 4.72 2.1% Sample 2 201 296 166 236 225 4.72 2.1% Sample 3 2403 2329 2427 2205 2341 163.17 7% Sample 3 2403 2329 2427 2205 2341 163.17 7%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 137 201 2403 127 163 2010 Standard deviation 9.55 9.27 160.76 5.82 11.8 98.49 CV (%) 7% 4.6% 6.7% 4.6% 7.2% 4.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 137 201 2403 127 163 2010 Standard deviation 9.55 9.27 160.76 5.82 11.8 98.49 CV (%) 7% 4.6% 6.7% 4.6% 7.2% 4.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 137 201 2403 127 163 2010 Mean (pg/ml) 137 201 2403 127 163 2010 Standard deviation 9.55 9.27 160.76 5.82 11.8 98.49 Standard deviation 9.55 9.27 160.76 5.82 11.8 98.49 CV (%) 7% 4.6% 6.7% 4.6% 7.2% 4.9% CV (%) 7% 4.6% 6.7% 4.6% 7.2% 4.9%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.073 0.167 0.225 0.369 0.6259 1.107 1.793 2.4 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.073 0.167 0.225 0.369 0.6259 1.107 1.793 2.4 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.073 0.167 0.225 0.369 0.6259 1.107 1.793 2.4 O.D. 0.073 0.167 0.225 0.369 0.6259 1.107 1.793 2.4

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.