Human LRIG1 ELISA Kit PicoKine®

Leucine-rich repeats and immunoglobulin-like domains protein 1 is a protein that in humans is encoded by the LRIG1 gene. It encodes atransmembrane proteinthat has been shown to interact with receptor tyrosine kinases of theEGFR-family, METandRET. Acts as a feedback negative regulator of signaling by receptor tyrosine kinases, through a mechanism that involves enhancement of receptor ubiquitination and accelerated intracellular degradation.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 44 37 42 45 42 1.76 4.2% Sample 2 70 73 124 117 96 4.32 4.5% Sample 3 885 953 982 700 880 25.17 2.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 44 37 42 45 42 1.76 4.2% Sample 1 44 37 42 45 42 1.76 4.2% Sample 2 70 73 124 117 96 4.32 4.5% Sample 2 70 73 124 117 96 4.32 4.5% Sample 3 885 953 982 700 880 25.17 2.9% Sample 3 885 953 982 700 880 25.17 2.9%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 44 70 885 47 83 924 Standard deviation 3.14 2.6 34.69 2.35 3.49 41.67 CV (%) 7.1% 3.7% 3.9% 5.0% 4.2% 4.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 44 70 885 47 83 924 Standard deviation 3.14 2.6 34.69 2.35 3.49 41.67 CV (%) 7.1% 3.7% 3.9% 5.0% 4.2% 4.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 44 70 885 47 83 924 Mean (pg/ml) 44 70 885 47 83 924 Standard deviation 3.14 2.6 34.69 2.35 3.49 41.67 Standard deviation 3.14 2.6 34.69 2.35 3.49 41.67 CV (%) 7.1% 3.7% 3.9% 5.0% 4.2% 4.5% CV (%) 7.1% 3.7% 3.9% 5.0% 4.2% 4.5%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.055 0.195 0.273 0.443 0.673 1.058 1.825 2.787 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.055 0.195 0.273 0.443 0.673 1.058 1.825 2.787 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.055 0.195 0.273 0.443 0.673 1.058 1.825 2.787 O.D. 0.055 0.195 0.273 0.443 0.673 1.058 1.825 2.787

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.