Human Lefty ELISA Kit PicoKine®

Left-right determination factor 1 is a protein that in humans is encoded by the LEFTY1 gene. This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate the mature protein, which plays a role in left-right asymmetry determination of organ systems during development. This gene is closely linked to both a related family member and a related pseudogene.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 28 22 24 22 24 1.51 6.3% Sample 2 59 33 58 38 47 3.48 7.4% Sample 3 449 473 380 375 419 29.01 6.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 28 22 24 22 24 1.51 6.3% Sample 1 28 22 24 22 24 1.51 6.3% Sample 2 59 33 58 38 47 3.48 7.4% Sample 2 59 33 58 38 47 3.48 7.4% Sample 3 449 473 380 375 419 29.01 6.9% Sample 3 449 473 380 375 419 29.01 6.9%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 28 56 449 25 58 354 Standard deviation 1.41 4.16 25.46 1.13 3.62 26.34 CV (%) 5% 7.1% 5.7% 4.5% 6.2% 7.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 28 56 449 25 58 354 Standard deviation 1.41 4.16 25.46 1.13 3.62 26.34 CV (%) 5% 7.1% 5.7% 4.5% 6.2% 7.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 28 56 449 25 58 354 Mean (pg/ml) 28 56 449 25 58 354 Standard deviation 1.41 4.16 25.46 1.13 3.62 26.34 Standard deviation 1.41 4.16 25.46 1.13 3.62 26.34 CV (%) 5% 7.1% 5.7% 4.5% 6.2% 7.4% CV (%) 5% 7.1% 5.7% 4.5% 6.2% 7.4%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.052 0.145 0.201 0.321 0.554 0.917 1.45 2.042 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.052 0.145 0.201 0.321 0.554 0.917 1.45 2.042 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.052 0.145 0.201 0.321 0.554 0.917 1.45 2.042 O.D. 0.052 0.145 0.201 0.321 0.554 0.917 1.45 2.042

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.