Human LECT2 ELISA Kit PicoKine®

This gene encodes a secreted, 16 kDa protein that acts as a chemotactic factor to neutrophils and stimulates the growth of chondrocytes and osteoblasts. This protein has high sequence similarity to the chondromodulin repeat regions of the chicken myb-induced myeloid 1 protein. A polymorphism in this gene may be associated with rheumatoid arthritis.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 32 38 52 45 42 2.22 5.3% Sample 2 98 79 106 108 98 3.76 3.9% Sample 3 966 728 930 937 890 51.46 5.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 32 38 52 45 42 2.22 5.3% Sample 1 32 38 52 45 42 2.22 5.3% Sample 2 98 79 106 108 98 3.76 3.9% Sample 2 98 79 106 108 98 3.76 3.9% Sample 3 966 728 930 937 890 51.46 5.8% Sample 3 966 728 930 937 890 51.46 5.8%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 32 98 966 53 69 748 Standard deviation 1.75 5.44 48.49 2.01 4.33 42.04 CV (%) 5.5% 5.6% 5% 3.8% 6.3% 5.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 32 98 966 53 69 748 Standard deviation 1.75 5.44 48.49 2.01 4.33 42.04 CV (%) 5.5% 5.6% 5% 3.8% 6.3% 5.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 32 98 966 53 69 748 Mean (pg/ml) 32 98 966 53 69 748 Standard deviation 1.75 5.44 48.49 2.01 4.33 42.04 Standard deviation 1.75 5.44 48.49 2.01 4.33 42.04 CV (%) 5.5% 5.6% 5% 3.8% 6.3% 5.6% CV (%) 5.5% 5.6% 5% 3.8% 6.3% 5.6%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.063 0.22 0.314 0.494 0.757 1.205 1.839 2.828 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.063 0.22 0.314 0.494 0.757 1.205 1.839 2.828 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.063 0.22 0.314 0.494 0.757 1.205 1.839 2.828 O.D. 0.063 0.22 0.314 0.494 0.757 1.205 1.839 2.828

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.