Human Galectin-8 ELISA Kit PicoKine®

Human Galectin-8 PicoKine® ELISA Kit (96 Tests) . Quantitate Human Galectin-8 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 25 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Galectin-8 is a protein of the galectin family that in humans is encoded by the LGALS8 gene. This gene encodes a member of the galectin family. Galectins are beta-galactoside-binding animal lectins with conserved carbohydrate recognition domains. The galectins have been implicated in many essential functions including development, differentiation, cell-cell adhesion, cell-matrix interaction, growth regulation, apoptosis, and RNA splicing. This gene is widely expressed in tumoral tissues and seems to be involved in integrin-like cell interactions. Alternatively spliced transcript variants encoding different isoforms have been identified.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 48 79 67 85 70 4.3 6.2% Sample 2 146 177 153 123 150 7.73 5.2% Sample 3 945 1007 955 1262 1042 73.69 7.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 48 79 67 85 70 4.3 6.2% Sample 1 48 79 67 85 70 4.3 6.2% Sample 2 146 177 153 123 150 7.73 5.2% Sample 2 146 177 153 123 150 7.73 5.2% Sample 3 945 1007 955 1262 1042 73.69 7.1% Sample 3 945 1007 955 1262 1042 73.69 7.1%

Gene Name

LGALS8

Gene ID

3964

UniProt

O00214

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: M1-W317

Capture Antibody

A polyclonal antibody from goat

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA)

Detection Range

46.9 pg/mL - 3,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 48 146 945 89 144 955 Standard deviation 2.07 6.58 25.04 2.6 10.87 28.75 CV (%) 4.3% 4.5% 2.7% 2.9% 7.6% 3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 48 146 945 89 144 955 Standard deviation 2.07 6.58 25.04 2.6 10.87 28.75 CV (%) 4.3% 4.5% 2.7% 2.9% 7.6% 3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 48 146 945 89 144 955 Mean (pg/ml) 48 146 945 89 144 955 Standard deviation 2.07 6.58 25.04 2.6 10.87 28.75 Standard deviation 2.07 6.58 25.04 2.6 10.87 28.75 CV (%) 4.3% 4.5% 2.7% 2.9% 7.6% 3% CV (%) 4.3% 4.5% 2.7% 2.9% 7.6% 3%

Sensitivity

<25 pg/mL

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human LGALS8

Anticoagulant

Heparin or EDTA

Sample Data

Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.052 0.153 0.27 0.448 0.762 1.27 1.87 2.2 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.052 0.153 0.27 0.448 0.762 1.27 1.87 2.2 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.052 0.153 0.27 0.448 0.762 1.27 1.87 2.2 O.D. 0.052 0.153 0.27 0.448 0.762 1.27 1.87 2.2

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.