Human LRG1 ELISA Kit PicoKine®

Leucine-rich alpha-2-glycoprotein 1 is a protein which in humans is encoded by the gene LRG1. The leucine-rich repeat (LRR) family of proteins, including LRG1, have been shown to be involved in protein-protein interaction, signal transduction, and cell adhesion and development. LRG1 is expressed during granulocyte differentiation.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1060 820 1217 1160 1064 60.77 5.7% Sample 2 2321 1411 1653 1516 1725 113.18 6.6% Sample 3 18753 15634 16014 17741 17036 1333.88 7.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1060 820 1217 1160 1064 60.77 5.7% Sample 1 1060 820 1217 1160 1064 60.77 5.7% Sample 2 2321 1411 1653 1516 1725 113.18 6.6% Sample 2 2321 1411 1653 1516 1725 113.18 6.6% Sample 3 18753 15634 16014 17741 17036 1333.88 7.8% Sample 3 18753 15634 16014 17741 17036 1333.88 7.8%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1060 2321 18753 626 1268 12203 Standard deviation 42.29 118.84 1177.69 14.27 91.42 541.81 CV (%) 4% 5.1% 6.3% 2.3% 7.2% 4.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1060 2321 18753 626 1268 12203 Standard deviation 42.29 118.84 1177.69 14.27 91.42 541.81 CV (%) 4% 5.1% 6.3% 2.3% 7.2% 4.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1060 2321 18753 626 1268 12203 Mean (pg/ml) 1060 2321 18753 626 1268 12203 Standard deviation 42.29 118.84 1177.69 14.27 91.42 541.81 Standard deviation 42.29 118.84 1177.69 14.27 91.42 541.81 CV (%) 4% 5.1% 6.3% 2.3% 7.2% 4.4% CV (%) 4% 5.1% 6.3% 2.3% 7.2% 4.4%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.021 0.105 0.161 0.289 0.557 1.003 1.685 2.351 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.021 0.105 0.161 0.289 0.557 1.003 1.685 2.351 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.021 0.105 0.161 0.289 0.557 1.003 1.685 2.351 O.D. 0.021 0.105 0.161 0.289 0.557 1.003 1.685 2.351

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.