Mouse Layilin ELISA Kit PicoKine®

Layilin is a protein that in humans is encoded by the LAYN gene. Layilin recently characterized as a 55 kDa transmembrane protein with homology to C-type lectins, is present in numerous cell lines and tissue extracts. As one of the adaptor proteins, talin mediates the interactions between the actin filaments and the cell membrane by binding to integral membrane proteins and the cytoskeleton. Layilin is a newly identified membrane-binding site for talin in peripheral ruffles of spreading cells, a ten-amino acid motif in the Layilin cytoplasmic domain is sufficient for talin binding, and its adjacent LH2-LH3 tandem arrays in the cytoplasmic domain provide docking sites for talin. Furthermore, talin binds Layilin, PIPK1gamma, and integrins in similar although subtly different ways. Layilin binds specifically to hyaluronan (HA) through its extracellular domain, a ubiquitous extracellular matrix component in most animal tissues and body fluids, but not to other tested glycosaminoglycans. The research suggests that Layilin may mediate signals from the extracellular matrix to the cell cytoskeleton via interaction with different intracellular binding partners and thereby be involved in the modulation of cortical structures in the cell. All the above actions reveal an interesting parallel between Layilin and the known HA receptor CD44. Also, merlin and radixin have been identified as different intracellular binding partners of Layilin. Accordingly, it has been suggested that Layilin plays roles in a variety of cellular processes, including cell shape, adhesion, motility, and homeostasis, as well as signal transduction. Besides, Layilin might play an important role in the process of invasion and lymphatic metastasis of lung carcinoma.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 264 276 272 310 281 19.8 7.1% Sample 2 568 449 388 320 431 11.82 2.7% Sample 3 4084 3288 3777 3018 3542 165.05 4.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 264 276 272 310 281 19.8 7.1% Sample 1 264 276 272 310 281 19.8 7.1% Sample 2 568 449 388 320 431 11.82 2.7% Sample 2 568 449 388 320 431 11.82 2.7% Sample 3 4084 3288 3777 3018 3542 165.05 4.7% Sample 3 4084 3288 3777 3018 3542 165.05 4.7%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 264 568 4084 168 567 4163 Standard deviation 13.36 31.52 177.25 10.35 43.49 180.26 CV (%) 5.1% 5.6% 4.3% 6.2% 7.7% 4.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 264 568 4084 168 567 4163 Standard deviation 13.36 31.52 177.25 10.35 43.49 180.26 CV (%) 5.1% 5.6% 4.3% 6.2% 7.7% 4.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 264 568 4084 168 567 4163 Mean (pg/ml) 264 568 4084 168 567 4163 Standard deviation 13.36 31.52 177.25 10.35 43.49 180.26 Standard deviation 13.36 31.52 177.25 10.35 43.49 180.26 CV (%) 5.1% 5.6% 4.3% 6.2% 7.7% 4.3% CV (%) 5.1% 5.6% 4.3% 6.2% 7.7% 4.3%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.023 0.096 0.167 0.294 0.548 0.961 1.641 2.534 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.023 0.096 0.167 0.294 0.548 0.961 1.641 2.534 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.023 0.096 0.167 0.294 0.548 0.961 1.641 2.534 O.D. 0.023 0.096 0.167 0.294 0.548 0.961 1.641 2.534

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.