Human CD107b ELISA Kit PicoKine®

Lysosome-associated membrane protein 2 (LAMP2), also known as CD107b (Cluster of Differentiation 107b) and Mac-3, is a human gene. The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may play a role in tumor cell metastasis. It may also function in the protection, maintenance, and adhesion of the lysosome. Alternative splicing of this gene results in multiple transcript variants encoding distinct proteins.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 515 623 533 605 569 19.18 3.4% Sample 2 810 777 1131 1175 973 55.57 5.7% Sample 3 7076 5285 6249 9727 7084 440.64 6.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 515 623 533 605 569 19.18 3.4% Sample 1 515 623 533 605 569 19.18 3.4% Sample 2 810 777 1131 1175 973 55.57 5.7% Sample 2 810 777 1131 1175 973 55.57 5.7% Sample 3 7076 5285 6249 9727 7084 440.64 6.2% Sample 3 7076 5285 6249 9727 7084 440.64 6.2%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 515 810 7076 395 638 6468 Standard deviation 22.81 26.73 235.63 22.99 40.83 355.09 CV (%) 4.4% 3.3% 3.3% 5.8% 6.4% 5.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 515 810 7076 395 638 6468 Standard deviation 22.81 26.73 235.63 22.99 40.83 355.09 CV (%) 4.4% 3.3% 3.3% 5.8% 6.4% 5.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 515 810 7076 395 638 6468 Mean (pg/ml) 515 810 7076 395 638 6468 Standard deviation 22.81 26.73 235.63 22.99 40.83 355.09 Standard deviation 22.81 26.73 235.63 22.99 40.83 355.09 CV (%) 4.4% 3.3% 3.3% 5.8% 6.4% 5.5% CV (%) 4.4% 3.3% 3.3% 5.8% 6.4% 5.5%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.025 0.121 0.199 0.326 0.6258 1.123 1.913 2.483 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.025 0.121 0.199 0.326 0.6258 1.123 1.913 2.483 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.025 0.121 0.199 0.326 0.6258 1.123 1.913 2.483 O.D. 0.025 0.121 0.199 0.326 0.6258 1.123 1.913 2.483

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.