Human CD107a ELISA Kit PicoKine®

Lysosomal-associated membrane protein 1 (LAMP-1) also known as lysosome-associated membrane glycoprotein 1 and CD107a (Cluster of Differentiation 107a), is a protein that in humans is encoded by the LAMP1 gene. The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may also play a role in tumor cell metastasis.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 574 513 455 584 532 16.8 3.2% Sample 2 899 902 733 982 879 47.64 5.4% Sample 3 5292 8796 6829 7925 7211 494.64 6.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 574 513 455 584 532 16.8 3.2% Sample 1 574 513 455 584 532 16.8 3.2% Sample 2 899 902 733 982 879 47.64 5.4% Sample 2 899 902 733 982 879 47.64 5.4% Sample 3 5292 8796 6829 7925 7211 494.64 6.9% Sample 3 5292 8796 6829 7925 7211 494.64 6.9%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 574 899 5292 315 1211 5536 Standard deviation 19.17 21.31 346.1 13.55 54.98 400.81 CV (%) 3.3% 2.4% 6.5% 4.3% 4.5% 7.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 574 899 5292 315 1211 5536 Standard deviation 19.17 21.31 346.1 13.55 54.98 400.81 CV (%) 3.3% 2.4% 6.5% 4.3% 4.5% 7.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 574 899 5292 315 1211 5536 Mean (pg/ml) 574 899 5292 315 1211 5536 Standard deviation 19.17 21.31 346.1 13.55 54.98 400.81 Standard deviation 19.17 21.31 346.1 13.55 54.98 400.81 CV (%) 3.3% 2.4% 6.5% 4.3% 4.5% 7.2% CV (%) 3.3% 2.4% 6.5% 4.3% 4.5% 7.2%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.01 0.073 0.138 0.255 0.465 0.802 1.384 2.096 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.01 0.073 0.138 0.255 0.465 0.802 1.384 2.096 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.01 0.073 0.138 0.255 0.465 0.802 1.384 2.096 O.D. 0.01 0.073 0.138 0.255 0.465 0.802 1.384 2.096

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.