Human Lipocalin-1 ELISA Kit PicoKine®

Lipocalin-1 is a protein that in humans is encoded by the LCN1 gene. This gene encodes a member of the lipocalin family of small secretory proteins. Lipocalins are extracellular transport proteins that bind to a variety of hydrophobic ligands. The encoded protein is the primary lipid binding protein in tears and is overproduced in response to multiple stimuli including infection and stress. The encoded protein may be a marker for chromosome aneuploidy as well as an autoantigen in Sjogren's syndrome. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and two pseudogenes of this gene are also located on the long arm of chromosome 9.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 239 213 234 221 227 17.28 7.6% Sample 2 467 451 478 326 431 11.97 2.8% Sample 3 3891 3425 3196 2685 3299 141.87 4.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 239 213 234 221 227 17.28 7.6% Sample 1 239 213 234 221 227 17.28 7.6% Sample 2 467 451 478 326 431 11.97 2.8% Sample 2 467 451 478 326 431 11.97 2.8% Sample 3 3891 3425 3196 2685 3299 141.87 4.3% Sample 3 3891 3425 3196 2685 3299 141.87 4.3%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 239 467 3891 188 427 3569 Standard deviation 9.61 12.24 264.2 14.25 31.38 267.68 CV (%) 4% 2.6% 6.8% 7.6% 7.4% 7.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 239 467 3891 188 427 3569 Standard deviation 9.61 12.24 264.2 14.25 31.38 267.68 CV (%) 4% 2.6% 6.8% 7.6% 7.4% 7.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 239 467 3891 188 427 3569 Mean (pg/ml) 239 467 3891 188 427 3569 Standard deviation 9.61 12.24 264.2 14.25 31.38 267.68 Standard deviation 9.61 12.24 264.2 14.25 31.38 267.68 CV (%) 4% 2.6% 6.8% 7.6% 7.4% 7.5% CV (%) 4% 2.6% 6.8% 7.6% 7.4% 7.5%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.005 0.063 0.124 0.226 0.371 0.726 1.376 2.198 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.005 0.063 0.124 0.226 0.371 0.726 1.376 2.198 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.005 0.063 0.124 0.226 0.371 0.726 1.376 2.198 O.D. 0.005 0.063 0.124 0.226 0.371 0.726 1.376 2.198

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.