Human CD125 ELISA Kit PicoKine®

Interleukin 5 receptor, alpha (IL5RA) also known as CD125 (Cluster of Differentiation 125) is a subunit of the Interleukin-5 receptor. IL5RA also denotes its human gene. The protein encoded by this gene is an interleukin 5 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5) . The binding of this protein to IL5 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL5. This protein has been found to interact with syndecan binding protein (syntenin), which is required for IL5 mediated activation of the transcription factor SOX4. Several alternatively spliced transcript variants encoding four distinct isoforms have been reported.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 34 58 59 35 47 3.24 7% Sample 2 67 122 73 113 94 3.94 4.2% Sample 3 678 613 963 724 745 31.86 4.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 34 58 59 35 47 3.24 7% Sample 1 34 58 59 35 47 3.24 7% Sample 2 67 122 73 113 94 3.94 4.2% Sample 2 67 122 73 113 94 3.94 4.2% Sample 3 678 613 963 724 745 31.86 4.3% Sample 3 678 613 963 724 745 31.86 4.3%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 34 67 678 49 95 886 Standard deviation 1.2 2.92 28.48 2.58 2.06 68.41 CV (%) 3.5% 4.4% 4.2% 5.3% 2.2% 7.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 34 67 678 49 95 886 Standard deviation 1.2 2.92 28.48 2.58 2.06 68.41 CV (%) 3.5% 4.4% 4.2% 5.3% 2.2% 7.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 34 67 678 49 95 886 Mean (pg/ml) 34 67 678 49 95 886 Standard deviation 1.2 2.92 28.48 2.58 2.06 68.41 Standard deviation 1.2 2.92 28.48 2.58 2.06 68.41 CV (%) 3.5% 4.4% 4.2% 5.3% 2.2% 7.9% CV (%) 3.5% 4.4% 4.2% 5.3% 2.2% 7.9%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.066 0.123 0.164 0.256 0.432 0.712 1.275 2.163 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.066 0.123 0.164 0.256 0.432 0.712 1.275 2.163 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.066 0.123 0.164 0.256 0.432 0.712 1.275 2.163 O.D. 0.066 0.123 0.164 0.256 0.432 0.712 1.275 2.163

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.