Human IL10RB ELISA Kit PicoKine®

Interleukin 10 receptor, beta subunit is a subunit for the interleukin-10 receptor. IL10RB is its human gene. The protein encoded by this gene belongs to the cytokine receptor family. It is an accessory chain essential for the active interleukin 10 receptor complex. Coexpression of this and IL10RA proteins has been shown to be required for IL10-induced signal transduction. This gene and three other interferon receptor genes, IFAR2, IFNAR1, and IFNGR2, form a class II cytokine receptor gene cluster located in a small region on chromosome 21.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 224 259 297 189 242 14.46 6% Sample 2 359 577 478 517 483 37.46 7.8% Sample 3 4418 2596 4568 2765 3587 258.96 7.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 224 259 297 189 242 14.46 6% Sample 1 224 259 297 189 242 14.46 6% Sample 2 359 577 478 517 483 37.46 7.8% Sample 2 359 577 478 517 483 37.46 7.8% Sample 3 4418 2596 4568 2765 3587 258.96 7.2% Sample 3 4418 2596 4568 2765 3587 258.96 7.2%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 224 359 4418 173 561 3368 Standard deviation 7.86 18.52 341.51 11.71 37.19 264.1 CV (%) 3.5% 5.2% 7.7% 6.8% 6.6% 7.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 224 359 4418 173 561 3368 Standard deviation 7.86 18.52 341.51 11.71 37.19 264.1 CV (%) 3.5% 5.2% 7.7% 6.8% 6.6% 7.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 224 359 4418 173 561 3368 Mean (pg/ml) 224 359 4418 173 561 3368 Standard deviation 7.86 18.52 341.51 11.71 37.19 264.1 Standard deviation 7.86 18.52 341.51 11.71 37.19 264.1 CV (%) 3.5% 5.2% 7.7% 6.8% 6.6% 7.2% CV (%) 3.5% 5.2% 7.7% 6.8% 6.6% 7.2%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.06 0.145 0.216 0.371 0.667 1.11 1.784 2.536 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.06 0.145 0.216 0.371 0.667 1.11 1.784 2.536 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.06 0.145 0.216 0.371 0.667 1.11 1.784 2.536 O.D. 0.06 0.145 0.216 0.371 0.667 1.11 1.784 2.536

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.