Human CD49A ELISA Kit PicoKine®

Integrin alpha-1 also CD49a is an integrin alpha subunit encoded in humans by the gene ITGA1. This gene encodes the alpha 1 subunit of integrin receptors. This protein heterodimerizes with the beta 1 subunit to form a cell-surface receptor for collagen and laminin. The heterodimeric receptor is involved in cell-cell adhesion and may play a role in inflammation and fibrosis. The alpha 1 subunit contains an inserted (I) von Willebrand factor type I domain which is thought to be involved in collagen binding.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 161 268 173 196 200 5.83 2.9% Sample 2 592 625 368 345 483 19.35 4% Sample 3 3907 3743 3150 4519 3830 288.76 7.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 161 268 173 196 200 5.83 2.9% Sample 1 161 268 173 196 200 5.83 2.9% Sample 2 592 625 368 345 483 19.35 4% Sample 2 592 625 368 345 483 19.35 4% Sample 3 3907 3743 3150 4519 3830 288.76 7.5% Sample 3 3907 3743 3150 4519 3830 288.76 7.5%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 161 592 3907 202 617 4838 Standard deviation 9.77 12.31 100.02 7.84 35.79 166.91 CV (%) 6.1% 2.1% 2.6% 3.9% 5.8% 3.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 161 592 3907 202 617 4838 Standard deviation 9.77 12.31 100.02 7.84 35.79 166.91 CV (%) 6.1% 2.1% 2.6% 3.9% 5.8% 3.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 161 592 3907 202 617 4838 Mean (pg/ml) 161 592 3907 202 617 4838 Standard deviation 9.77 12.31 100.02 7.84 35.79 166.91 Standard deviation 9.77 12.31 100.02 7.84 35.79 166.91 CV (%) 6.1% 2.1% 2.6% 3.9% 5.8% 3.5% CV (%) 6.1% 2.1% 2.6% 3.9% 5.8% 3.5%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.057 0.167 0.258 0.429 0.731 1.252 1.852 2.416 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.057 0.167 0.258 0.429 0.731 1.252 1.852 2.416 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.057 0.167 0.258 0.429 0.731 1.252 1.852 2.416 O.D. 0.057 0.167 0.258 0.429 0.731 1.252 1.852 2.416

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.