Human Jagged1 ELISA Kit PicoKine®

The jagged 1 protein encoded by JAG1 is the human homolog of the Drosophilia jagged protein. Human jagged 1 is the ligand for the receptor notch 1, the latter is involved in signaling processes. Mutations that alter the jagged 1 protein cause Alagille syndrome. Jagged 1 signalling through notch 1 has also been shown to play a role in hematopoiesis.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 90 96 75 67 82 4.44 5.4% Sample 2 185 143 239 169 184 12.6 6.9% Sample 3 1704 1270 1116 1693 1446 93.40 6.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 90 96 75 67 82 4.44 5.4% Sample 1 90 96 75 67 82 4.44 5.4% Sample 2 185 143 239 169 184 12.6 6.9% Sample 2 185 143 239 169 184 12.6 6.9% Sample 3 1704 1270 1116 1693 1446 93.40 6.5% Sample 3 1704 1270 1116 1693 1446 93.40 6.5%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 90 185 1704 76 200 1687 Standard deviation 2.79 12.91 48.39 4.92 10.12 69.34 CV (%) 3.1% 7% 2.8% 6.5% 5.1% 4.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 90 185 1704 76 200 1687 Standard deviation 2.79 12.91 48.39 4.92 10.12 69.34 CV (%) 3.1% 7% 2.8% 6.5% 5.1% 4.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 90 185 1704 76 200 1687 Mean (pg/ml) 90 185 1704 76 200 1687 Standard deviation 2.79 12.91 48.39 4.92 10.12 69.34 Standard deviation 2.79 12.91 48.39 4.92 10.12 69.34 CV (%) 3.1% 7% 2.8% 6.5% 5.1% 4.1% CV (%) 3.1% 7% 2.8% 6.5% 5.1% 4.1%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.032 0.085 0.117 0.19 0.333 0.592 1.04 1.785 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.032 0.085 0.117 0.19 0.333 0.592 1.04 1.785 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.032 0.085 0.117 0.19 0.333 0.592 1.04 1.785 O.D. 0.032 0.085 0.117 0.19 0.333 0.592 1.04 1.785

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.