Human CD51 ELISA Kit PicoKine®

Integrin alpha-V is a protein that in humans is encoded by the ITGAV gene. The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha subunit and a beta subunit that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha V subunit. This subunit associates with beta 1, beta 3, beta 5, beta 6 and beta 8 subunits. The heterodimer consisting of alpha V and beta 3 subunits is also known as the vitronectin receptor. This integrin may regulate angiogenesis and cancer progression. Alternative splicing results in multiple transcript variants. Note that the integrin alpha 5 and integrin alpha V subunits are encoded by distinct genes.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 179 151 95 120 136 3.15 2.3% Sample 2 236 204 241 308 247 12.81 5.2% Sample 3 2233 1878 2332 2092 2134 93.24 4.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 179 151 95 120 136 3.15 2.3% Sample 1 179 151 95 120 136 3.15 2.3% Sample 2 236 204 241 308 247 12.81 5.2% Sample 2 236 204 241 308 247 12.81 5.2% Sample 3 2233 1878 2332 2092 2134 93.24 4.4% Sample 3 2233 1878 2332 2092 2134 93.24 4.4%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 179 236 2233 108 249 2866 Standard deviation 6.01 14.18 86.64 4.36 19 95.15 CV (%) 3.4% 6% 3.9% 4% 7.6% 3.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 179 236 2233 108 249 2866 Standard deviation 6.01 14.18 86.64 4.36 19 95.15 CV (%) 3.4% 6% 3.9% 4% 7.6% 3.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 179 236 2233 108 249 2866 Mean (pg/ml) 179 236 2233 108 249 2866 Standard deviation 6.01 14.18 86.64 4.36 19 95.15 Standard deviation 6.01 14.18 86.64 4.36 19 95.15 CV (%) 3.4% 6% 3.9% 4% 7.6% 3.3% CV (%) 3.4% 6% 3.9% 4% 7.6% 3.3%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.038 0.114 0.184 0.286 0.532 0.957 1.577 2.508 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.038 0.114 0.184 0.286 0.532 0.957 1.577 2.508 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.038 0.114 0.184 0.286 0.532 0.957 1.577 2.508 O.D. 0.038 0.114 0.184 0.286 0.532 0.957 1.577 2.508

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.