Human CD94 ELISA Kit PicoKine®

CD94 (Cluster of Differentiation 94), also known as killer cell lectin-like receptor subfamily D, member 1 (KLRD1) is a human gene. Natural killer (NK) cells are a distinct lineage of lymphocytes that mediate cytotoxic activity and secrete cytokines upon immune stimulation. Several genes of the C-type lectin superfamily, including members of the NKG2 family, are expressed by NK cells and may be involved in the regulation of NK cell function. KLRD1 (CD94) is an antigen preferentially expressed on NK cells and is classified as a type II membrane protein because it has an external C terminus. Several transcript variants encoding different isoforms have been found for this gene.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 79 66 80 62 72 2.55 3.6% Sample 2 125 148 150 179 151 10.14 6.7% Sample 3 924 1005 1015 1430 1094 36.96 3.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 79 66 80 62 72 2.55 3.6% Sample 1 79 66 80 62 72 2.55 3.6% Sample 2 125 148 150 179 151 10.14 6.7% Sample 2 125 148 150 179 151 10.14 6.7% Sample 3 924 1005 1015 1430 1094 36.96 3.4% Sample 3 924 1005 1015 1430 1094 36.96 3.4%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 78 172 1200 67 172 1212 Standard deviation 3.29 12.83 87.24 2.3 11.27 63.15 CV (%) 4.2% 7.5% 7.3% 3.4% 6.6% 5.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 78 172 1200 67 172 1212 Standard deviation 3.29 12.83 87.24 2.3 11.27 63.15 CV (%) 4.2% 7.5% 7.3% 3.4% 6.6% 5.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 78 172 1200 67 172 1212 Mean (pg/ml) 78 172 1200 67 172 1212 Standard deviation 3.29 12.83 87.24 2.3 11.27 63.15 Standard deviation 3.29 12.83 87.24 2.3 11.27 63.15 CV (%) 4.2% 7.5% 7.3% 3.4% 6.6% 5.2% CV (%) 4.2% 7.5% 7.3% 3.4% 6.6% 5.2%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.038 0.109 0.184 0.306 0.532 0.957 1.637 2.508 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.038 0.109 0.184 0.306 0.532 0.957 1.637 2.508 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.038 0.109 0.184 0.306 0.532 0.957 1.637 2.508 O.D. 0.038 0.109 0.184 0.306 0.532 0.957 1.637 2.508

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.