Human CD11b ELISA Kit PicoKine®

This gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3') . The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 796 785 804 812 799 20.78 2.6% Sample 2 2799 2647 2860 2971 2819 87.4 3.1% Sample 3 6548 6972 7069 7798 7097 283.87 4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 796 785 804 812 799 20.78 2.6% Sample 1 796 785 804 812 799 20.78 2.6% Sample 2 2799 2647 2860 2971 2819 87.4 3.1% Sample 2 2799 2647 2860 2971 2819 87.4 3.1% Sample 3 6548 6972 7069 7798 7097 283.87 4% Sample 3 6548 6972 7069 7798 7097 283.87 4%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 796 2799 6548 748 2864 6697 Standard deviation 48.56 190.33 353.59 36.65 160.38 361.64 CV (%) 6.1% 6.8% 5.4% 4.9% 5.6% 5.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 796 2799 6548 748 2864 6697 Standard deviation 48.56 190.33 353.59 36.65 160.38 361.64 CV (%) 6.1% 6.8% 5.4% 4.9% 5.6% 5.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 796 2799 6548 748 2864 6697 Mean (pg/ml) 796 2799 6548 748 2864 6697 Standard deviation 48.56 190.33 353.59 36.65 160.38 361.64 Standard deviation 48.56 190.33 353.59 36.65 160.38 361.64 CV (%) 6.1% 6.8% 5.4% 4.9% 5.6% 5.4% CV (%) 6.1% 6.8% 5.4% 4.9% 5.6% 5.4%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.03 0.111 0.176 0.288 0.524 0.949 1.569 2.5 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.03 0.111 0.176 0.288 0.524 0.949 1.569 2.5 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.03 0.111 0.176 0.288 0.524 0.949 1.569 2.5 O.D. 0.03 0.111 0.176 0.288 0.524 0.949 1.569 2.5

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.