Human HS6ST1 ELISA Kit PicoKine®

The protein encoded by this gene is a member of the heparan sulfate biosynthetic enzyme family. Heparan sulfate biosynthetic enzymes are key components in generating a myriad of distinct heparan sulfate fine structures that carry out multiple biological activities. This enzyme is a type II integral membrane protein and is responsible for 6-O-sulfation of heparan sulfate. This enzyme does not share significant sequence similarity with other known sulfotransferases. A pseudogene located on chromosome 1 has been found for this gene.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 22 21 18 24 21 0.7 3.3% Sample 2 189 174 163 178 176 7.92 4.5% Sample 3 477 455 436 492 465 27.44 5.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 22 21 18 24 21 0.7 3.3% Sample 1 22 21 18 24 21 0.7 3.3% Sample 2 189 174 163 178 176 7.92 4.5% Sample 2 189 174 163 178 176 7.92 4.5% Sample 3 477 455 436 492 465 27.44 5.9% Sample 3 477 455 436 492 465 27.44 5.9%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 22 189 477 29 176 424 Standard deviation 1.19 12.29 32.91 1.51 9.33 26.29 CV (%) 5.4% 6.5% 6.9% 5.2% 5.3% 6.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 22 189 477 29 176 424 Standard deviation 1.19 12.29 32.91 1.51 9.33 26.29 CV (%) 5.4% 6.5% 6.9% 5.2% 5.3% 6.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 22 189 477 29 176 424 Mean (pg/ml) 22 189 477 29 176 424 Standard deviation 1.19 12.29 32.91 1.51 9.33 26.29 Standard deviation 1.19 12.29 32.91 1.51 9.33 26.29 CV (%) 5.4% 6.5% 6.9% 5.2% 5.3% 6.2% CV (%) 5.4% 6.5% 6.9% 5.2% 5.3% 6.2%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.011 0.083 0.157 0.279 0.505 0.93 1.55 2.425 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.011 0.083 0.157 0.279 0.505 0.93 1.55 2.425 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.011 0.083 0.157 0.279 0.505 0.93 1.55 2.425 O.D. 0.011 0.083 0.157 0.279 0.505 0.93 1.55 2.425

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.