Human ICAM4 ELISA Kit PicoKine®

This gene encodes the Landsteiner-Wiener (LW) blood group antigen (s) that belongs to the immunoglobulin (Ig) superfamily, and that shares similarity with the intercellular adhesion molecule (ICAM) protein family. This ICAM protein contains 2 Ig-like C2-type domains and binds to the leukocyte adhesion LFA-1 protein. The molecular basis of the LW (A) /LW (B) blood group antigens is a single aa variation at position 100; Gln-100=LW (A) and Arg-100=LW (B) . Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 49 45 39 47 45 2.66 5.9% Sample 2 163 146 159 170 160 7.5 4.7% Sample 3 653 632 644 679 652 36.51 5.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 49 45 39 47 45 2.66 5.9% Sample 1 49 45 39 47 45 2.66 5.9% Sample 2 163 146 159 170 160 7.5 4.7% Sample 2 163 146 159 170 160 7.5 4.7% Sample 3 653 632 644 679 652 36.51 5.6% Sample 3 653 632 644 679 652 36.51 5.6%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 49 163 653 52 176 774 Standard deviation 3.43 10.6 38.53 3.02 9.5 42.57 CV (%) 7% 6.5% 5.9% 5.8% 5.4% 5.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 49 163 653 52 176 774 Standard deviation 3.43 10.6 38.53 3.02 9.5 42.57 CV (%) 7% 6.5% 5.9% 5.8% 5.4% 5.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 49 163 653 52 176 774 Mean (pg/ml) 49 163 653 52 176 774 Standard deviation 3.43 10.6 38.53 3.02 9.5 42.57 Standard deviation 3.43 10.6 38.53 3.02 9.5 42.57 CV (%) 7% 6.5% 5.9% 5.8% 5.4% 5.5% CV (%) 7% 6.5% 5.9% 5.8% 5.4% 5.5%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.006 0.166 0.274 0.43 0.745 1.205 1.966 2.976 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.006 0.166 0.274 0.43 0.745 1.205 1.966 2.976 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.006 0.166 0.274 0.43 0.745 1.205 1.966 2.976 O.D. 0.006 0.166 0.274 0.43 0.745 1.205 1.966 2.976

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.