Human B7-H2/ICOSL ELISA Kit PicoKine®

Human B7-H2/ICOSL PicoKine® ELISA Kit (96 Tests) . Quantitate Human B7-H2/ICOSL in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 300 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

ICOS ligand (ICOSLG, ICOSL or GL50) is a protein that in humans is encoded by the ICOSLG gene located at chromosome 21. Enables identical protein binding activity. Predicted to be involved in T cell receptor signaling pathway and positive regulation of interleukin-4 production. Located in cytoplasmic ribonucleoprotein granule and plasma membrane.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 2015 2162 1984 1947 2027 99.32 4.9% Sample 2 4613 4696 4536 4799 4661 270.34 5.8% Sample 3 23647 25479 24519 22796 24020 1393.17 5.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 2015 2162 1984 1947 2027 99.32 4.9% Sample 1 2015 2162 1984 1947 2027 99.32 4.9% Sample 2 4613 4696 4536 4799 4661 270.34 5.8% Sample 2 4613 4696 4536 4799 4661 270.34 5.8% Sample 3 23647 25479 24519 22796 24020 1393.17 5.8% Sample 3 23647 25479 24519 22796 24020 1393.17 5.8%

Gene Name

ICOSLG

Gene ID

23308

UniProt

O75144

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: D19-S258

Capture Antibody

A polyclonal antibody from goat

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Standard

40 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA)

Detection Range

625 pg/mL - 40,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 2015 4613 23467 1987 4563 21547 Standard deviation 90.68 281.39 1608 97.36 333.1 1335.91 CV (%) 4.5% 6.1% 6.8% 4.9% 7.3% 6.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 2015 4613 23467 1987 4563 21547 Standard deviation 90.68 281.39 1608 97.36 333.1 1335.91 CV (%) 4.5% 6.1% 6.8% 4.9% 7.3% 6.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 2015 4613 23467 1987 4563 21547 Mean (pg/ml) 2015 4613 23467 1987 4563 21547 Standard deviation 90.68 281.39 1608 97.36 333.1 1335.91 Standard deviation 90.68 281.39 1608 97.36 333.1 1335.91 CV (%) 4.5% 6.1% 6.8% 4.9% 7.3% 6.2% CV (%) 4.5% 6.1% 6.8% 4.9% 7.3% 6.2%

Sensitivity

<300 pg/mL

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human ICOSLG

Anticoagulant

Heparin or EDTA

Sample Data

Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.042 0.117 0.188 0.32 0.556 0.961 1.681 2.612 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.042 0.117 0.188 0.32 0.556 0.961 1.681 2.612 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.042 0.117 0.188 0.32 0.556 0.961 1.681 2.612 O.D. 0.042 0.117 0.188 0.32 0.556 0.961 1.681 2.612

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.