Human SDF-1/CXCL12 ELISA Kit PicoKine®

The stromal cell-derived factor 1 (SDF-1), also known as C-X-C motif chemokine 12 (CXCL12), is a chemokine protein that in humans is encoded by the CXCL12 gene on chromosome 10. This antimicrobial gene encodes a stromal cell-derived alpha chemokine member of the intercrine family. The encoded protein functions as the ligand for the G-protein coupled receptor, chemokine (C-X-C motif) receptor 4, and plays a role in many diverse cellular functions, including embryogenesis, immune surveillance, inflammation response, tissue homeostasis, and tumor growth and metastasis. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 112 108 105 125 113 3.26 2.9% Sample 2 498 466 452 467 471 14.59 3.1% Sample 3 1863 1769 1845 1766 1811 61.57 3.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 112 108 105 125 113 3.26 2.9% Sample 1 112 108 105 125 113 3.26 2.9% Sample 2 498 466 452 467 471 14.59 3.1% Sample 2 498 466 452 467 471 14.59 3.1% Sample 3 1863 1769 1845 1766 1811 61.57 3.4% Sample 3 1863 1769 1845 1766 1811 61.57 3.4%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 112 498 1863 122 479 1863 Standard deviation 4.93 20.42 96.88 3.9 22.03 72.66 CV (%) 4.4% 4.1% 5.2% 3.2% 4.6% 3.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 112 498 1863 122 479 1863 Standard deviation 4.93 20.42 96.88 3.9 22.03 72.66 CV (%) 4.4% 4.1% 5.2% 3.2% 4.6% 3.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 112 498 1863 122 479 1863 Mean (pg/ml) 112 498 1863 122 479 1863 Standard deviation 4.93 20.42 96.88 3.9 22.03 72.66 Standard deviation 4.93 20.42 96.88 3.9 22.03 72.66 CV (%) 4.4% 4.1% 5.2% 3.2% 4.6% 3.9% CV (%) 4.4% 4.1% 5.2% 3.2% 4.6% 3.9%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.038 0.18 0.293 0.45 0.737 1.148 1.668 2.048 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.038 0.18 0.293 0.45 0.737 1.148 1.668 2.048 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.038 0.18 0.293 0.45 0.737 1.148 1.668 2.048 O.D. 0.038 0.18 0.293 0.45 0.737 1.148 1.668 2.048

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.