Human HNMT ELISA Kit PicoKine®

In mammals, histamine is metabolized by two major pathways: N (tau) -methylation via histamine N-methyltransferase and oxidative deamination via diamine oxidase. This gene encodes the first enzyme which is found in the cytosol and uses S-adenosyl-L-methionine as the methyl donor. In the mammalian brain, the neurotransmitter activity of histamine is controlled by N (tau) -methylation as diamine oxidase is not found in the central nervous system. A common genetic polymorphism affects the activity levels of this gene product in red blood cells. Multiple alternatively spliced transcript variants that encode different proteins have been found for this gene.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 113 121 118 109 115 4.15 6.1% Sample 2 684 721 706 698 702 42.84 6.1% Sample 3 1463 1522 1469 1463 1479 72.48 4.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 113 121 118 109 115 4.15 6.1% Sample 1 113 121 118 109 115 4.15 6.1% Sample 2 684 721 706 698 702 42.84 6.1% Sample 2 684 721 706 698 702 42.84 6.1% Sample 3 1463 1522 1469 1463 1479 72.48 4.9% Sample 3 1463 1522 1469 1463 1479 72.48 4.9%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 113 684 1463 110 702 1532 Standard deviation 4.41 34.88 70.22 5.72 31.59 78.13 CV (%) 3.9% 5.1% 4.8% 5.2% 4.5% 5.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 113 684 1463 110 702 1532 Standard deviation 4.41 34.88 70.22 5.72 31.59 78.13 CV (%) 3.9% 5.1% 4.8% 5.2% 4.5% 5.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 113 684 1463 110 702 1532 Mean (pg/ml) 113 684 1463 110 702 1532 Standard deviation 4.41 34.88 70.22 5.72 31.59 78.13 Standard deviation 4.41 34.88 70.22 5.72 31.59 78.13 CV (%) 3.9% 5.1% 4.8% 5.2% 4.5% 5.1% CV (%) 3.9% 5.1% 4.8% 5.2% 4.5% 5.1%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.039 0.145 0.231 0.382 0.649 1.038 1.615 1.828 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.039 0.145 0.231 0.382 0.649 1.038 1.615 1.828 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.039 0.145 0.231 0.382 0.649 1.038 1.615 1.828 O.D. 0.039 0.145 0.231 0.382 0.649 1.038 1.615 1.828

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.