Human GUSB ELISA Kit PicoKine®

This gene encodes a hydrolase that degrades glycosaminoglycans, including heparan sulfate, dermatan sulfate, and chondroitin-4,6-sulfate. The enzyme forms a homotetramer that is localized to the lysosome. Mutations in this gene result in mucopolysaccharidosis type VII. Alternative splicing results in multiple transcript variants. There are many pseudogenes of this locus in the human genome.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 362 363 367 372 366 15.74 4.3% Sample 2 1379 1368 1385 1296 1357 74.64 5.5% Sample 3 4926 4593 4768 4852 4785 282.30 5.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 362 363 367 372 366 15.74 4.3% Sample 1 362 363 367 372 366 15.74 4.3% Sample 2 1379 1368 1385 1296 1357 74.64 5.5% Sample 2 1379 1368 1385 1296 1357 74.64 5.5% Sample 3 4926 4593 4768 4852 4785 282.30 5.9% Sample 3 4926 4593 4768 4852 4785 282.30 5.9%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 362 1379 4926 388 1295 4782 Standard deviation 19.91 70.33 241.37 16.68 62.16 186.5 CV (%) 5.5% 5.1% 4.9% 4.3% 4.8% 3.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 362 1379 4926 388 1295 4782 Standard deviation 19.91 70.33 241.37 16.68 62.16 186.5 CV (%) 5.5% 5.1% 4.9% 4.3% 4.8% 3.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 362 1379 4926 388 1295 4782 Mean (pg/ml) 362 1379 4926 388 1295 4782 Standard deviation 19.91 70.33 241.37 16.68 62.16 186.5 Standard deviation 19.91 70.33 241.37 16.68 62.16 186.5 CV (%) 5.5% 5.1% 4.9% 4.3% 4.8% 3.9% CV (%) 5.5% 5.1% 4.9% 4.3% 4.8% 3.9%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.049 0.107 0.165 0.261 0.461 0.83 1.424 2.269 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.049 0.107 0.165 0.261 0.461 0.83 1.424 2.269 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.049 0.107 0.165 0.261 0.461 0.83 1.424 2.269 O.D. 0.049 0.107 0.165 0.261 0.461 0.83 1.424 2.269

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.