Human GSTM1 ELISA Kit PicoKine®

Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-transferase that belongs to the mu class. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding the mu class of enzymes are organized in a gene cluster on chromosome 1p13.3 and are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of certain drugs. Null mutations of this class mu gene have been linked with an increase in a number of cancers, likely due to an increased susceptibility to environmental toxins and carcinogens. Multiple protein isoforms are encoded by transcript variants of this gene.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 86 88 91 87 88 3.17 3.6% Sample 2 397 348 392 368 376 10.91 2.9% Sample 3 712 689 693 716 703 27.4 3.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 86 88 91 87 88 3.17 3.6% Sample 1 86 88 91 87 88 3.17 3.6% Sample 2 397 348 392 368 376 10.91 2.9% Sample 2 397 348 392 368 376 10.91 2.9% Sample 3 712 689 693 716 703 27.4 3.9% Sample 3 712 689 693 716 703 27.4 3.9%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 86 397 712 102 421 788 Standard deviation 4.39 17.87 42.01 3.98 18.95 40.19 CV (%) 5.1% 4.5% 5.9% 3.9% 4.5% 5.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 86 397 712 102 421 788 Standard deviation 4.39 17.87 42.01 3.98 18.95 40.19 CV (%) 5.1% 4.5% 5.9% 3.9% 4.5% 5.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 86 397 712 102 421 788 Mean (pg/ml) 86 397 712 102 421 788 Standard deviation 4.39 17.87 42.01 3.98 18.95 40.19 Standard deviation 4.39 17.87 42.01 3.98 18.95 40.19 CV (%) 5.1% 4.5% 5.9% 3.9% 4.5% 5.1% CV (%) 5.1% 4.5% 5.9% 3.9% 4.5% 5.1%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.004 0.081 0.13 0.254 0.484 0.892 1.615 2.281 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.004 0.081 0.13 0.254 0.484 0.892 1.615 2.281 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.004 0.081 0.13 0.254 0.484 0.892 1.615 2.281 O.D. 0.004 0.081 0.13 0.254 0.484 0.892 1.615 2.281

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.