Human GPVI ELISA Kit PicoKine®

Glycoprotein VI (platelet), also known as GPVI, is a glycoprotein receptor for collagen which is expressed in platelets. In humans, glycoprotein VI is encoded by the GPVI gene. This gene encodes a platelet membrane glycoprotein of the immunoglobulin superfamily. The encoded protein is a receptor for collagen and plays a critical role in collagen-induced platelet aggregation and thrombus formation. The encoded protein forms a complex with the Fc receptor gamma-chain that initiates the platelet activation signaling cascade upon collagen binding. Mutations in this gene are a cause of platelet-type bleeding disorder-11 (BDPLT11) . Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 23 17 17 17 19 0.42 2.3% Sample 2 112 122 95 124 113 8.69 7.7% Sample 3 361 476 331 423 398 28.24 7.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 23 17 17 17 19 0.42 2.3% Sample 1 23 17 17 17 19 0.42 2.3% Sample 2 112 122 95 124 113 8.69 7.7% Sample 2 112 122 95 124 113 8.69 7.7% Sample 3 361 476 331 423 398 28.24 7.1% Sample 3 361 476 331 423 398 28.24 7.1%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 23 112 361 16 67 261 Standard deviation 1.81 6.23 26.24 0.62 4.25 7.31 CV (%) 7.9% 5.6% 7.3% 3.9% 6.4% 2.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 23 112 361 16 67 261 Standard deviation 1.81 6.23 26.24 0.62 4.25 7.31 CV (%) 7.9% 5.6% 7.3% 3.9% 6.4% 2.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 23 112 361 16 67 261 Mean (pg/ml) 23 112 361 16 67 261 Standard deviation 1.81 6.23 26.24 0.62 4.25 7.31 Standard deviation 1.81 6.23 26.24 0.62 4.25 7.31 CV (%) 7.9% 5.6% 7.3% 3.9% 6.4% 2.8% CV (%) 7.9% 5.6% 7.3% 3.9% 6.4% 2.8%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.095 0.184 0.261 0.418 0.7 1.181 1.923 2.508 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.095 0.184 0.261 0.418 0.7 1.181 1.923 2.508 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.095 0.184 0.261 0.418 0.7 1.181 1.923 2.508 O.D. 0.095 0.184 0.261 0.418 0.7 1.181 1.923 2.508

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.