Human CD42d ELISA Kit PicoKine®

Glycoprotein V (platelet) (GP5) also known as CD42d (Cluster of Differentiation 42d), is a human gene. Human platelet glycoprotein V (GP5) is a part of the Ib-V-IX system of surface glycoproteins that constitute the receptor for von Willebrand factor (VWF; MIM 613160) and mediate the adhesion of platelets to injured vascular surfaces in the arterial circulation, a critical initiating event in hemostasis. The main portion of the receptor is a heterodimer composed of 2 polypeptide chains, an alpha chain (GP1BA; MIM 606672) and a beta chain (GP1BB; MIM 138720), that are linked by disulfide bonds. The complete receptor complex includes noncovalent association of the alpha and beta subunits with platelet glycoprotein IX (GP9; MIM 173515) and GP5. Mutations in GP1BA, GP1BB, and GP9 have been shown to cause Bernard-Soulier syndrome, a bleeding disorde.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 108 122 117 125 118 7.2 6.1% Sample 2 466 462 501 481 478 26.26 5.5% Sample 3 1635 1752 1693 1727 1702 100.4 5.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 108 122 117 125 118 7.2 6.1% Sample 1 108 122 117 125 118 7.2 6.1% Sample 2 466 462 501 481 478 26.26 5.5% Sample 2 466 462 501 481 478 26.26 5.5% Sample 3 1635 1752 1693 1727 1702 100.4 5.9% Sample 3 1635 1752 1693 1727 1702 100.4 5.9%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 108 466 1635 118 474 1588 Standard deviation 5.51 20.97 78.48 4.6 19.43 76.22 CV (%) 5.1% 4.5% 4.8% 3.9% 4.1% 4.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 108 466 1635 118 474 1588 Standard deviation 5.51 20.97 78.48 4.6 19.43 76.22 CV (%) 5.1% 4.5% 4.8% 3.9% 4.1% 4.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 108 466 1635 118 474 1588 Mean (pg/ml) 108 466 1635 118 474 1588 Standard deviation 5.51 20.97 78.48 4.6 19.43 76.22 Standard deviation 5.51 20.97 78.48 4.6 19.43 76.22 CV (%) 5.1% 4.5% 4.8% 3.9% 4.1% 4.8% CV (%) 5.1% 4.5% 4.8% 3.9% 4.1% 4.8%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.035 0.148 0.247 0.411 0.745 1.32 1.997 2.608 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.035 0.148 0.247 0.411 0.745 1.32 1.997 2.608 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.035 0.148 0.247 0.411 0.745 1.32 1.997 2.608 O.D. 0.035 0.148 0.247 0.411 0.745 1.32 1.997 2.608

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.