Human CD42b ELISA Kit PicoKine®

Platelet glycoprotein Ib alpha chain also known as glycoprotein Ib (platelet), alpha polypeptide or CD42b (Cluster of Differentiation 42b), is a protein that in humans is encoded by the GP1BA gene. Glycoprotein Ib (GP Ib) is a platelet surface membrane glycoprotein composed of a heterodimer, an alpha chain and a beta chain, that is linked by disulfide bonds. The Gp Ib functions as a receptor for von Willebrand factor (VWF) . The complete receptor complex includes noncovalent association of the alpha and beta subunits with platelet glycoprotein IX and platelet glycoprotein V. The binding of the GP Ib-IX-V complex to VWF facilitates initial platelet adhesion to vascular subendothelium after vascular injury, and also initiates signaling events within the platelet that lead to enhanced platelet activation, thrombosis, and hemostasis. This gene encodes the alpha subunit. Mutations in this gene result in Bernard-Soulier syndromes and platelet-type von Willebrand disease. The coding region of this gene is known to contain a polymophic variable number tandem repeat (VNTR) domain that is associated with susceptibility to nonarteritic anterior ischemic optic neuropathy.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 264 247 219 251 245 9.32 3.8% Sample 2 1499 1455 1324 1378 1414 50.9 3.6% Sample 3 5696 5411 5439 5282 5457 223.74 4.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 264 247 219 251 245 9.32 3.8% Sample 1 264 247 219 251 245 9.32 3.8% Sample 2 1499 1455 1324 1378 1414 50.9 3.6% Sample 2 1499 1455 1324 1378 1414 50.9 3.6% Sample 3 5696 5411 5439 5282 5457 223.74 4.1% Sample 3 5696 5411 5439 5282 5457 223.74 4.1%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 264 1499 5696 259 1432 5590 Standard deviation 14.52 77.95 205.06 13.21 64.44 268.32 CV (%) 5.5% 5.2% 3.6% 5.1% 4.5% 4.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 264 1499 5696 259 1432 5590 Standard deviation 14.52 77.95 205.06 13.21 64.44 268.32 CV (%) 5.5% 5.2% 3.6% 5.1% 4.5% 4.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 264 1499 5696 259 1432 5590 Mean (pg/ml) 264 1499 5696 259 1432 5590 Standard deviation 14.52 77.95 205.06 13.21 64.44 268.32 Standard deviation 14.52 77.95 205.06 13.21 64.44 268.32 CV (%) 5.5% 5.2% 3.6% 5.1% 4.5% 4.8% CV (%) 5.5% 5.2% 3.6% 5.1% 4.5% 4.8%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.006 0.059 0.114 0.208 0.398 0.706 1.264 2.075 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.006 0.059 0.114 0.208 0.398 0.706 1.264 2.075 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.006 0.059 0.114 0.208 0.398 0.706 1.264 2.075 O.D. 0.006 0.059 0.114 0.208 0.398 0.706 1.264 2.075

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.