Human GLB1 ELISA Kit PicoKine®

Galactosidase, beta 1, also known as GLB1, is a protein which in humans is encoded by the GLB1 gene. This gene encodes a member of the glycosyl hydrolase 35 family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature lysosomal enzyme. This enzyme catalyzes the hydrolysis of a terminal beta-linked galactose residue from ganglioside substrates and other glycoconjugates. Mutations in this gene may result in GM1-gangliosidosis and Morquio B syndrome.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 141 138 155 146 145 8.27 5.7% Sample 2 862 866 880 822 858 45.45 5.3% Sample 3 3964 3564 3841 3952 3830 222.15 5.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 141 138 155 146 145 8.27 5.7% Sample 1 141 138 155 146 145 8.27 5.7% Sample 2 862 866 880 822 858 45.45 5.3% Sample 2 862 866 880 822 858 45.45 5.3% Sample 3 3964 3564 3841 3952 3830 222.15 5.8% Sample 3 3964 3564 3841 3952 3830 222.15 5.8%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 141 862 3964 138 756 3785 Standard deviation 9.02 37.93 273.52 8 44.6 196.82 CV (%) 6.4% 4.4% 6.9% 5.8% 5.9% 5.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 141 862 3964 138 756 3785 Standard deviation 9.02 37.93 273.52 8 44.6 196.82 CV (%) 6.4% 4.4% 6.9% 5.8% 5.9% 5.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 141 862 3964 138 756 3785 Mean (pg/ml) 141 862 3964 138 756 3785 Standard deviation 9.02 37.93 273.52 8 44.6 196.82 Standard deviation 9.02 37.93 273.52 8 44.6 196.82 CV (%) 6.4% 4.4% 6.9% 5.8% 5.9% 5.2% CV (%) 6.4% 4.4% 6.9% 5.8% 5.9% 5.2%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.023 0.105 0.192 0.355 0.5777 1.062 1.734 2.166 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.023 0.105 0.192 0.355 0.5777 1.062 1.734 2.166 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.023 0.105 0.192 0.355 0.5777 1.062 1.734 2.166 O.D. 0.023 0.105 0.192 0.355 0.5777 1.062 1.734 2.166

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.